An Ultrasensitive Protein Detection Technology Based on Aptamer Binding and Exponential Amplicfication Using Cross-Catalytic DNAzyme Reactions

We have designed a novel system for ultra-senstivie protein detection. This technology utilized DNA Strand displacement through aptamer binding. The signal for the detection of a trace amount of protein in solution is exponentially amplified by cross-catalytic DNAzyme cleavage reactions. In brief, a targe protein will bind to a DNA aptamer sequence and trigger a cross-catalytic reaction. The exponential cleavage of cicularized DNA oligos into linear oligos will be detected by a nanoparticle based colorimetric detection scheme. To ensure a major and signifcant commerical advatage over other protein detection systems, the most significant advantage of our proposed technology is that the detection device is highly portable; also it possesses the additional protperites:1. Isothermal reactions2. Enzyme free exponential amplifications3. versatility with the capability of detectiong unlimited type of proteinsThe successful implementation of this innovative technology will be revolutionary for exquistely sensitive cancer marker diagnostics.