An ultra-rapid cryo-technique for complex organisms

T. Irdani, Angelo Fortunato, R. Torre

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The soil nematode Caenorhabditis elegans is an excellent research model in cell biology, human disease and developmental studies. In this study, a novel cryopreservation technique based on a rapid cooling procedure, previously established for juveniles, was applied to adult-worms. Here we demonstrated that adults of C. elegans, a complex metazoan organism, survive to a rapid cooling and storage in liquid nitrogen (-196 °C) with a very high survival percentage (85%). The procedure relies on a Low CryoProtectant Technique (LCPT) and Ultra Rapid Cooling (URC). The high cooling rate is achieved through the reduction of sample volumes and the effectiveness of a nylon carrier. Our technique complies with the requirements for vitrification to occur. The main distinctive characters of this cryopreservation technique compared to other methods, e.g. Slow Freezing and Vitrification, are presented. Our results show that this cryopreservation method is valid for both unicellular and multicellular organisms; it is suitable for short or long time storage in liquid-nitrogen. This technique promises to be a unique and simpler method for cryostorage of cells, organisms and tissues.

Original languageEnglish (US)
Pages (from-to)391-397
Number of pages7
JournalCryobiology
Volume71
Issue number3
DOIs
StatePublished - Dec 1 2015

Fingerprint

Cooling
Cryopreservation
Vitrification
organisms
Liquid nitrogen
Caenorhabditis elegans
cooling
Cytology
cryopreservation
Nitrogen
Nylons
vitrification
methodology
Freezing
Cell Biology
Tissue
Soils
Soil
soil nematodes
liquids

Keywords

  • C. elegans
  • Cooling-rate
  • Cryopreservation
  • Long-term storage
  • Low cryoprotectant technique
  • Organisms cryostorage
  • Thermal-analysis
  • Vitrification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

An ultra-rapid cryo-technique for complex organisms. / Irdani, T.; Fortunato, Angelo; Torre, R.

In: Cryobiology, Vol. 71, No. 3, 01.12.2015, p. 391-397.

Research output: Contribution to journalArticle

Irdani, T. ; Fortunato, Angelo ; Torre, R. / An ultra-rapid cryo-technique for complex organisms. In: Cryobiology. 2015 ; Vol. 71, No. 3. pp. 391-397.
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