An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors

Rhett L. Martineau, Bruce C. Towe, Valerie Stout

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

One well-developed paradigm for biosensing uses living microorganisms as sensors for environmental stimuli. In this paradigm, engineered cells contain plasmid or chromosomal sequences that link stress-inducible promoters to the production of fluorescent reporter proteins. We are developing such a sensor system with an aim for portability. This work describes the development and performance of a compact optical system designed for low-power detection of fluorescent reporter proteins in microliter-scale cell suspensions. Light-emitting diodes (LEDs) and silicon photodetectors (PDs) were configured to measure red fluorescence, green fluorescence, and cell biomass pseudosimultaneously in 100 μl samples. The optical detectors were calibrated using E. coli cells that expressed red and green fluorescent proteins (dsRed2 and gfp-asv, Clontech) either constitutively or through chemical induction. We show that sufficient sensitivity for certain whole-cell biosensor applications is achievable in low-volume samples, despite the simplicity and low-cost nature of the detector system. The prototype optical detector occupies approximately 1.8 cm x 2.2 cm x 3.0 cm.

Original languageEnglish (US)
Title of host publication2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems
Pages90-93
Number of pages4
DOIs
StatePublished - Dec 1 2006
Event2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems, BMN - San Francisco, CA, United States
Duration: Jan 15 2006Jan 18 2006

Publication series

Name2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems

Other

Other2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems, BMN
CountryUnited States
CitySan Francisco, CA
Period1/15/061/18/06

ASJC Scopus subject areas

  • Biomedical Engineering
  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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    Martineau, R. L., Towe, B. C., & Stout, V. (2006). An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors. In 2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems (pp. 90-93). [4129433] (2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems). https://doi.org/10.1109/BMN.2006.330891