An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors

Rhett L. Martineau, Bruce C. Towe, Valerie Stout

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

One well-developed paradigm for biosensing uses living microorganisms as sensors for environmental stimuli. In this paradigm, engineered cells contain plasmid or chromosomal sequences that link stress-inducible promoters to the production of fluorescent reporter proteins. We are developing such a sensor system with an aim for portability. This work describes the development and performance of a compact optical system designed for low-power detection of fluorescent reporter proteins in microliter-scale cell suspensions. Light-emitting diodes (LEDs) and silicon photodetectors (PDs) were configured to measure red fluorescence, green fluorescence, and cell biomass pseudosimultaneously in 100 μl samples. The optical detectors were calibrated using E. coli cells that expressed red and green fluorescent proteins (dsRed2 and gfp-asv, Clontech) either constitutively or through chemical induction. We show that sufficient sensitivity for certain whole-cell biosensor applications is achievable in low-volume samples, despite the simplicity and low-cost nature of the detector system. The prototype optical detector occupies approximately 1.8 cm x 2.2 cm x 3.0 cm.

Original languageEnglish (US)
Title of host publication2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems
Pages90-93
Number of pages4
DOIs
StatePublished - 2006
Event2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems, BMN - San Francisco, CA, United States
Duration: Jan 15 2006Jan 18 2006

Other

Other2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems, BMN
CountryUnited States
CitySan Francisco, CA
Period1/15/061/18/06

Fingerprint

Biosensors
Detectors
Proteins
Fluorescence
Sensors
Photodetectors
Optical systems
Microorganisms
Escherichia coli
Light emitting diodes
Biomass
Silicon
Costs

ASJC Scopus subject areas

  • Biomedical Engineering
  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Martineau, R. L., Towe, B. C., & Stout, V. (2006). An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors. In 2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems (pp. 90-93). [4129433] https://doi.org/10.1109/BMN.2006.330891

An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors. / Martineau, Rhett L.; Towe, Bruce C.; Stout, Valerie.

2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems. 2006. p. 90-93 4129433.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Martineau, RL, Towe, BC & Stout, V 2006, An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors. in 2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems., 4129433, pp. 90-93, 2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems, BMN, San Francisco, CA, United States, 1/15/06. https://doi.org/10.1109/BMN.2006.330891
Martineau RL, Towe BC, Stout V. An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors. In 2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems. 2006. p. 90-93. 4129433 https://doi.org/10.1109/BMN.2006.330891
Martineau, Rhett L. ; Towe, Bruce C. ; Stout, Valerie. / An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors. 2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems. 2006. pp. 90-93
@inproceedings{074dd88d23694af9b1d61e7bc4a74f10,
title = "An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors",
abstract = "One well-developed paradigm for biosensing uses living microorganisms as sensors for environmental stimuli. In this paradigm, engineered cells contain plasmid or chromosomal sequences that link stress-inducible promoters to the production of fluorescent reporter proteins. We are developing such a sensor system with an aim for portability. This work describes the development and performance of a compact optical system designed for low-power detection of fluorescent reporter proteins in microliter-scale cell suspensions. Light-emitting diodes (LEDs) and silicon photodetectors (PDs) were configured to measure red fluorescence, green fluorescence, and cell biomass pseudosimultaneously in 100 μl samples. The optical detectors were calibrated using E. coli cells that expressed red and green fluorescent proteins (dsRed2 and gfp-asv, Clontech) either constitutively or through chemical induction. We show that sufficient sensitivity for certain whole-cell biosensor applications is achievable in low-volume samples, despite the simplicity and low-cost nature of the detector system. The prototype optical detector occupies approximately 1.8 cm x 2.2 cm x 3.0 cm.",
author = "Martineau, {Rhett L.} and Towe, {Bruce C.} and Valerie Stout",
year = "2006",
doi = "10.1109/BMN.2006.330891",
language = "English (US)",
isbn = "1424400562",
pages = "90--93",
booktitle = "2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems",

}

TY - GEN

T1 - An optical micro-instrumentation system for measurement of fluorescent proteins in whole-cell biosensors

AU - Martineau, Rhett L.

AU - Towe, Bruce C.

AU - Stout, Valerie

PY - 2006

Y1 - 2006

N2 - One well-developed paradigm for biosensing uses living microorganisms as sensors for environmental stimuli. In this paradigm, engineered cells contain plasmid or chromosomal sequences that link stress-inducible promoters to the production of fluorescent reporter proteins. We are developing such a sensor system with an aim for portability. This work describes the development and performance of a compact optical system designed for low-power detection of fluorescent reporter proteins in microliter-scale cell suspensions. Light-emitting diodes (LEDs) and silicon photodetectors (PDs) were configured to measure red fluorescence, green fluorescence, and cell biomass pseudosimultaneously in 100 μl samples. The optical detectors were calibrated using E. coli cells that expressed red and green fluorescent proteins (dsRed2 and gfp-asv, Clontech) either constitutively or through chemical induction. We show that sufficient sensitivity for certain whole-cell biosensor applications is achievable in low-volume samples, despite the simplicity and low-cost nature of the detector system. The prototype optical detector occupies approximately 1.8 cm x 2.2 cm x 3.0 cm.

AB - One well-developed paradigm for biosensing uses living microorganisms as sensors for environmental stimuli. In this paradigm, engineered cells contain plasmid or chromosomal sequences that link stress-inducible promoters to the production of fluorescent reporter proteins. We are developing such a sensor system with an aim for portability. This work describes the development and performance of a compact optical system designed for low-power detection of fluorescent reporter proteins in microliter-scale cell suspensions. Light-emitting diodes (LEDs) and silicon photodetectors (PDs) were configured to measure red fluorescence, green fluorescence, and cell biomass pseudosimultaneously in 100 μl samples. The optical detectors were calibrated using E. coli cells that expressed red and green fluorescent proteins (dsRed2 and gfp-asv, Clontech) either constitutively or through chemical induction. We show that sufficient sensitivity for certain whole-cell biosensor applications is achievable in low-volume samples, despite the simplicity and low-cost nature of the detector system. The prototype optical detector occupies approximately 1.8 cm x 2.2 cm x 3.0 cm.

UR - http://www.scopus.com/inward/record.url?scp=50249172807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50249172807&partnerID=8YFLogxK

U2 - 10.1109/BMN.2006.330891

DO - 10.1109/BMN.2006.330891

M3 - Conference contribution

AN - SCOPUS:50249172807

SN - 1424400562

SN - 9781424400560

SP - 90

EP - 93

BT - 2nd ASM-IEEE EMBS Conference on Bio-, Micro- and Nanosystems

ER -