Investigation of the triplet state of flavines and flavoproteins by optical detection of magnetic resonance

Thomas A. Moore; Alvin L. Kwiram

doi:10.1021/bi00723a025

Investigation of the triplet state of flavines and flavoproteins by optical detection of magnetic resonance

Thomas A. Moore, Alvin L. Kwiram

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The magnetic sublevels of the lowest excited triplet state of riboflavine, deazaflavine, FMN, FAD, and alloxazine, as well as two flavoproteins have been investigated by optical detection of magnetic resonance. Lifetimes, relative steady-state populations, relative radiative rate constants, as well as zero-field splitting parameters are reported for riboflavine and deazaflavine. This double resonance technique has been used to observe and characterize phosphorescence from flavodoxin and yeast hemoglobin and is shown to be a powerful technique for investigating the triplet electronic structure of weakly luminescent biological systems.

Original language	English (US)
Pages (from-to)	5403-5407
Number of pages	5
Journal	Biochemistry
Volume	13
Issue number	26
DOIs	https://doi.org/10.1021/bi00723a025
State	Published - Dec 1 1974
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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10.1021/bi00723a025

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title = "Investigation of the triplet state of flavines and flavoproteins by optical detection of magnetic resonance",

abstract = "The magnetic sublevels of the lowest excited triplet state of riboflavine, deazaflavine, FMN, FAD, and alloxazine, as well as two flavoproteins have been investigated by optical detection of magnetic resonance. Lifetimes, relative steady-state populations, relative radiative rate constants, as well as zero-field splitting parameters are reported for riboflavine and deazaflavine. This double resonance technique has been used to observe and characterize phosphorescence from flavodoxin and yeast hemoglobin and is shown to be a powerful technique for investigating the triplet electronic structure of weakly luminescent biological systems.",

author = "Moore, {Thomas A.} and Kwiram, {Alvin L.}",

year = "1974",

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doi = "10.1021/bi00723a025",

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volume = "13",

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publisher = "American Chemical Society",

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T1 - Investigation of the triplet state of flavines and flavoproteins by optical detection of magnetic resonance

AU - Moore, Thomas A.

AU - Kwiram, Alvin L.

PY - 1974/12/1

Y1 - 1974/12/1

N2 - The magnetic sublevels of the lowest excited triplet state of riboflavine, deazaflavine, FMN, FAD, and alloxazine, as well as two flavoproteins have been investigated by optical detection of magnetic resonance. Lifetimes, relative steady-state populations, relative radiative rate constants, as well as zero-field splitting parameters are reported for riboflavine and deazaflavine. This double resonance technique has been used to observe and characterize phosphorescence from flavodoxin and yeast hemoglobin and is shown to be a powerful technique for investigating the triplet electronic structure of weakly luminescent biological systems.

AB - The magnetic sublevels of the lowest excited triplet state of riboflavine, deazaflavine, FMN, FAD, and alloxazine, as well as two flavoproteins have been investigated by optical detection of magnetic resonance. Lifetimes, relative steady-state populations, relative radiative rate constants, as well as zero-field splitting parameters are reported for riboflavine and deazaflavine. This double resonance technique has been used to observe and characterize phosphorescence from flavodoxin and yeast hemoglobin and is shown to be a powerful technique for investigating the triplet electronic structure of weakly luminescent biological systems.

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JO - Biochemistry

JF - Biochemistry

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ER -

Investigation of the triplet state of flavines and flavoproteins by optical detection of magnetic resonance

Abstract

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