An integrated system for continuous assessment of intercellular pH

Hany Arafa, Sahil Shah, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work we present a system that is capable of autonomously recording the pH of cultured cells in an incubator. The system is composed of an ion sensitive field effect transistor (ISFET), a transimpedance amplifier circuit, and a BeagleBone Black® computer which records ISFET output measurements. Cultured cells were grown directly on top of the ISFET, and the current output was converted to voltage via a transimpedance amplifier circuit. These converted voltage measurements were then recorded at user specified sampling rate. The system is completely self-contained with no need to run wires or cables from the inside of the incubator to the outside. We have demonstrated the system by observing cancer cell (human adenocarcinoma) proliferation over a 20 hour testing period.

Original languageEnglish (US)
Title of host publicationIEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages332-335
Number of pages4
ISBN (Print)9781479923465
DOIs
StatePublished - Dec 9 2014
Event10th IEEE Biomedical Circuits and Systems Conference, BioCAS 2014 - Lausanne, Switzerland
Duration: Oct 22 2014Oct 24 2014

Other

Other10th IEEE Biomedical Circuits and Systems Conference, BioCAS 2014
CountrySwitzerland
CityLausanne
Period10/22/1410/24/14

Fingerprint

Ion sensitive field effect transistors
Operational amplifiers
Cells
Networks (circuits)
Voltage measurement
Cables
Wire
Sampling
Testing
Electric potential

ASJC Scopus subject areas

  • Hardware and Architecture
  • Biomedical Engineering

Cite this

Arafa, H., Shah, S., & Blain Christen, J. (2014). An integrated system for continuous assessment of intercellular pH. In IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings (pp. 332-335). [6981730] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/BioCAS.2014.6981730

An integrated system for continuous assessment of intercellular pH. / Arafa, Hany; Shah, Sahil; Blain Christen, Jennifer.

IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2014. p. 332-335 6981730.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Arafa, H, Shah, S & Blain Christen, J 2014, An integrated system for continuous assessment of intercellular pH. in IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings., 6981730, Institute of Electrical and Electronics Engineers Inc., pp. 332-335, 10th IEEE Biomedical Circuits and Systems Conference, BioCAS 2014, Lausanne, Switzerland, 10/22/14. https://doi.org/10.1109/BioCAS.2014.6981730
Arafa H, Shah S, Blain Christen J. An integrated system for continuous assessment of intercellular pH. In IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2014. p. 332-335. 6981730 https://doi.org/10.1109/BioCAS.2014.6981730
Arafa, Hany ; Shah, Sahil ; Blain Christen, Jennifer. / An integrated system for continuous assessment of intercellular pH. IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 332-335
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