@article{c5d83a2abbc748e69fbad12d242918d3,
title = "An evolutionary perspective of DNA methylation patterns in skeletal tissues using a baboon model of osteoarthritis",
abstract = "Epigenetic factors, such as DNA methylation, play an influential role in the development of the degenerative joint disease osteoarthritis (OA). These molecular mechanisms have been heavily studied in humans, and although OA affects several other animals in addition to humans, few efforts have taken an evolutionary perspective. This study explores the evolution of OA epigenetics by assessing the relationship between DNA methylation variation and knee OA development in baboons (Papio spp.) and by comparing these findings to human OA epigenetic associations. Genome-wide DNA methylation patterns were identified in bone and cartilage of the right distal femora from 56 pedigreed, adult baboons (28 with and 28 without knee OA) using the Illumina Infinium MethylationEPIC BeadChip. Several significantly differentially methylated positions (DMPs) and regions were found between tissue types. Substantial OA-related differential methylation was also identified in cartilage, but not in bone, suggesting that cartilage epigenetics may be more influential in OA than bone epigenetics. Additionally, some genes containing OA-related DMPs overlap with and display methylation patterns similar to those previously identified in human OA, revealing a mixture of evolutionarily conserved and divergent OA-related methylation patterns in primates. Overall, these findings reinforce the current etiological perspectives of OA and enhance our evolutionary understanding of epigenetic mechanisms associated with OA. This study further establishes baboons as a valuable nonhuman primate model of OA, and continued investigations in baboons will help to disentangle the molecular mechanisms contributing to OA and their evolutionary histories.",
keywords = "animal model, bone, cartilage, epigenetics, osteoarthritis",
author = "Genevieve Housman and Quillen, {Ellen E.} and Stone, {Anne C.}",
note = "Funding Information: We thank Eric D. Johnson and members of the Department of Genetics at the Texas Biomedical Research Institute, including Anthony G. Comuzzie, Lorena M. Havill, Anne Sheldrake, Jaydee Foster, Kara Peterson, Mel Carless, and Laura Cox, for helpful discussions. This study was supported by the National Institutes of Health (P01HL028972 to Anthony G. Comuzzie); the Leakey Foundation (Research Grant for Doctoral Students to Genevieve Housman); the Wenner‐Gren Foundation (Gr. 9310 to Genevieve Housman); the Nacey Maggioncalda Foundation (James F. Nacey Fellowship to Genevieve Housman); the International Primatological Society (to Genevieve Housman); Sigma Xi (Grant‐in‐Aid of Research to Genevieve Housman); the ASU Center for Evolution and Medicine (Venture Fund to Genevieve Housman); and the ASU Graduate Research and Support Program (to Genevieve Housman). Additionally, this investigation used resources that were supported by the Southwest National Primate Research Center grant P51OD011133 from the Office of Research Infrastructure Programs, National Institutes of Health. Funding Information: We thank Eric D. Johnson and members of the Department of Genetics at the Texas Biomedical Research Institute, including Anthony G. Comuzzie, Lorena M. Havill, Anne Sheldrake, Jaydee Foster, Kara Peterson, Mel Carless, and Laura Cox, for helpful discussions. This study was supported by the National Institutes of Health (P01HL028972 to Anthony G. Comuzzie); the Leakey Foundation (Research Grant for Doctoral Students to Genevieve Housman); the Wenner-Gren Foundation (Gr. 9310 to Genevieve Housman); the Nacey Maggioncalda Foundation (James F. Nacey Fellowship to Genevieve Housman); the International Primatological Society (to Genevieve Housman); Sigma Xi (Grant-in-Aid of Research to Genevieve Housman); the ASU Center for Evolution and Medicine (Venture Fund to Genevieve Housman); and the ASU Graduate Research and Support Program (to Genevieve Housman). Additionally, this investigation used resources that were supported by the Southwest National Primate Research Center grant P51OD011133 from the Office of Research Infrastructure Programs, National Institutes of Health. Publisher Copyright: {\textcopyright} 2020 Orthopaedic Research Society. Published by Wiley Periodicals LLC",
year = "2021",
month = oct,
doi = "10.1002/jor.24957",
language = "English (US)",
volume = "39",
pages = "2260--2269",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "10",
}