TY - GEN
T1 - An escherichia coli concentrator using magnetic particles in a microfluidic channel for the urinary tract infection (UTI) application
AU - Yang, Yongmo
AU - Kim, Sangpyeong
AU - Chae, Junseok
N1 - Funding Information:
The authors thank to Mr. Seokheun Choi for numerous and valuable discussions, and staff in CSSER (Center for Solid-State Electronics Research) at Arizona State University. This work is partially supported by NSF (ECCS-0901440).
Publisher Copyright:
© 2010TRF.
PY - 2010
Y1 - 2010
N2 - This paper reports a miniaturized lab-on-a-chip device to detect Escherichia coli (E. coli) from simulated urine using magnetic particles (MPs) in a microfluidic channel. The lab-on-a-chip device consists of two chambers connected in series, E. coli concentration and sensing chamber, integrated with a label free impedance sensor to detect the concentration of E. coli for urinary tract infection (UTI) application. The two chamber configuration is designed to reduce potential false-positive response caused by proteins in urine. The integrated impedance sensor in the concentration chamber shows little impedance change in different E. coli concentrations due to masking effect by proteins while the one in the sensing chamber shows a significant (30 kΩ) change between control (no E. coli) and a concentration of 6.4x104 CFU/mL, close to the threshold of UTI infection, which is above 105 CFU/mL. This demonstrates the effectiveness of the dual-chamber design to detect E. coli for the UTI application.
AB - This paper reports a miniaturized lab-on-a-chip device to detect Escherichia coli (E. coli) from simulated urine using magnetic particles (MPs) in a microfluidic channel. The lab-on-a-chip device consists of two chambers connected in series, E. coli concentration and sensing chamber, integrated with a label free impedance sensor to detect the concentration of E. coli for urinary tract infection (UTI) application. The two chamber configuration is designed to reduce potential false-positive response caused by proteins in urine. The integrated impedance sensor in the concentration chamber shows little impedance change in different E. coli concentrations due to masking effect by proteins while the one in the sensing chamber shows a significant (30 kΩ) change between control (no E. coli) and a concentration of 6.4x104 CFU/mL, close to the threshold of UTI infection, which is above 105 CFU/mL. This demonstrates the effectiveness of the dual-chamber design to detect E. coli for the UTI application.
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U2 - 10.31438/trf.hh2010.44
DO - 10.31438/trf.hh2010.44
M3 - Conference contribution
AN - SCOPUS:85061787100
T3 - Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop
SP - 162
EP - 165
BT - 2010 Solid-State Sensors, Actuators, and Microsystems Workshop
A2 - Monk, David J.
A2 - Turner, Kimberly L.
PB - Transducer Research Foundation
T2 - 2010 Solid-State Sensors, Actuators, and Microsystems Workshop
Y2 - 6 June 2010 through 10 June 2010
ER -