An efficient plant viral expression system generating orally immunogenic Norwalk virus-like particles

Luca Santi, Lance Batchelor, Zhong Huang, Brooke Hjelm, Jacquelyn Kilbourne, Charles J. Arntzen, Qiang Chen, Hugh Mason

Research output: Contribution to journalArticle

136 Scopus citations

Abstract

Virus-like particles (VLPs) derived from enteric pathogens like Norwalk virus (NV) are well suited to study oral immunization. We previously described stable transgenic plants that accumulate recombinant NV-like particles (rNVs) that were orally immunogenic in mice and humans. The transgenic approach suffers from long generation time and modest level of antigen accumulation. We now overcome these constraints with an efficient tobacco mosaic virus (TMV)-derived transient expression system using leaves of Nicotiana benthamiana. We produced properly assembled rNV at 0.8 mg/g leaf 12 days post-infection (dpi). Oral immunization of CD1 mice with 100 or 250 μg/dose of partially purified rNV elicited systemic and mucosal immune responses. We conclude that the plant viral transient expression system provides a robust research tool to generate abundant quantities of rNV as enriched, concentrated VLP preparations that are orally immunogenic.

Original languageEnglish (US)
Pages (from-to)1846-1854
Number of pages9
JournalVaccine
Volume26
Issue number15
DOIs
StatePublished - Mar 28 2008

Keywords

  • Mucosal vaccination
  • Plant viral vector
  • Virus-like particles

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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