An E. coli gene coding for a protamine-like protein

Sidney Altman, Peter Model, Gordon H. Dixon, Michael A. Wosnick

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Several open reading frames exist in the region of the tRNA1 Tyr gene of the E. coli genome. One such sequence encodes a polypeptide 33 amino acids in length that, on the basis of size and amino acid sequence, bears a striking resemblance to the protamines found in trout sperm. DNA from the transducing phage φ80 tRNA1 Tyr su3+ directs in vitro synthesis of two small basic proteins that are not made when homologous DNA containing a deletion that overlaps both the su3+ gene and the gene for the putative protamine-like protein is used. DNA from phage that have regained the parental su3+ phenotype again direct in vitro synthesis of the basic proteins. Synthesis of basic protein is inhibited by the presence of ppGpp, as would be expected if the mRNA is part of a large RNA transcript that starts at the promoter for the tRNA1 Tyr gene.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalCell
Volume26
Issue number3 PART 1
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Protamines
Escherichia coli
Genes
Bacteriophages
DNA
Proteins
Trout
Amino Acids
Open Reading Frames
Spermatozoa
Amino Acid Sequence
Genome
RNA
Phenotype
Messenger RNA
Peptides
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Altman, S., Model, P., Dixon, G. H., & Wosnick, M. A. (1981). An E. coli gene coding for a protamine-like protein. Cell, 26(3 PART 1), 299-304. https://doi.org/10.1016/0092-8674(81)90198-7

An E. coli gene coding for a protamine-like protein. / Altman, Sidney; Model, Peter; Dixon, Gordon H.; Wosnick, Michael A.

In: Cell, Vol. 26, No. 3 PART 1, 1981, p. 299-304.

Research output: Contribution to journalArticle

Altman, S, Model, P, Dixon, GH & Wosnick, MA 1981, 'An E. coli gene coding for a protamine-like protein', Cell, vol. 26, no. 3 PART 1, pp. 299-304. https://doi.org/10.1016/0092-8674(81)90198-7
Altman, Sidney ; Model, Peter ; Dixon, Gordon H. ; Wosnick, Michael A. / An E. coli gene coding for a protamine-like protein. In: Cell. 1981 ; Vol. 26, No. 3 PART 1. pp. 299-304.
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