An ATP-Grasp ligase involved in the last biosynthetic step of the iminomycosporine shinorine in nostoc punctiforme ATCC 29133

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Abstract

We investigated the genetic basis for mycosporine sunscreen biosynthesis by the cyanobacterium Nostoc punctiforme ATCC 29133. Heterologous expression in Escherichia coli of three contiguous N. punctiforme genes (NpR5600, NpR5599, and NpR5598, here named mysA, mysB, and mysC, respectively) led to the production of mycosporine-glycine, an oxomycosporine. Additional expression of gene NpF5597 (mysD) led to the conversion of mycosporine-glycine into iminomycosporines (preferentially shinorine but also others like mycosporine-2- glycine and porphyra-334). This represents a new mode of enzymatic synthesis for iminomycosporines, one that differs in genetic origin, mechanism, and apparent substrate specificity from that known in Anabaena variabilis ATCC 29413. These results add to the emerging profile of the protein family of ATP-dependent ligases, to which the mysC product belongs, as important condensation enzymes in microbial secondary metabolism.

Original languageEnglish (US)
Pages (from-to)5923-5928
Number of pages6
JournalJournal of bacteriology
Volume193
Issue number21
DOIs
StatePublished - Nov 2011

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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