An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system

Ankita Shastri, Lynn M. McGregor, Ya Liu, Valerie Harris, Hanqing Nan, Maritza Mujica, Yolanda Vasquez, Amitabh Bhattacharya, Yongting Ma, Michael Aizenberg, Olga Kuksenok, Anna C. Balazs, Joanna Aizenberg, Ximin He

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

The efficient extraction of (bio)molecules from fluid mixtures is vital for applications ranging from target characterization in (bio)chemistry to environmental analysis and biomedical diagnostics. Inspired by biological processes that seamlessly synchronize the capture, transport and release of biomolecules, we designed a robust chemomechanical sorting system capable of the concerted catch and release of target biomolecules from a solution mixture. The hybrid system is composed of target-specific, reversible binding sites attached to microscopic fins embedded in a responsive hydrogel that moves the cargo between two chemically distinct environments. To demonstrate the utility of the system, we focus on the effective separation of thrombin by synchronizing the pH-dependent binding strength of a thrombin-specific aptamer with volume changes of the pH-responsive hydrogel in a biphasic microfluidic regime, and show a non-destructive separation that has a quantitative sorting efficiency, as well as the system's stability and amenability to multiple solution recycling.

Original languageEnglish (US)
Pages (from-to)447-454
Number of pages8
JournalNature Chemistry
Volume7
Issue number5
DOIs
StatePublished - May 1 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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    Shastri, A., McGregor, L. M., Liu, Y., Harris, V., Nan, H., Mujica, M., Vasquez, Y., Bhattacharya, A., Ma, Y., Aizenberg, M., Kuksenok, O., Balazs, A. C., Aizenberg, J., & He, X. (2015). An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system. Nature Chemistry, 7(5), 447-454. https://doi.org/10.1038/nchem.2203