An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system

Ankita Shastri, Lynn M. McGregor, Ya Liu, Valerie Harris, Hanqing Nan, Maritza Mujica, Yolanda Vasquez, Amitabh Bhattacharya, Yongting Ma, Michael Aizenberg, Olga Kuksenok, Anna C. Balazs, Joanna Aizenberg, Ximin He

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The efficient extraction of (bio)molecules from fluid mixtures is vital for applications ranging from target characterization in (bio)chemistry to environmental analysis and biomedical diagnostics. Inspired by biological processes that seamlessly synchronize the capture, transport and release of biomolecules, we designed a robust chemomechanical sorting system capable of the concerted catch and release of target biomolecules from a solution mixture. The hybrid system is composed of target-specific, reversible binding sites attached to microscopic fins embedded in a responsive hydrogel that moves the cargo between two chemically distinct environments. To demonstrate the utility of the system, we focus on the effective separation of thrombin by synchronizing the pH-dependent binding strength of a thrombin-specific aptamer with volume changes of the pH-responsive hydrogel in a biphasic microfluidic regime, and show a non-destructive separation that has a quantitative sorting efficiency, as well as the system's stability and amenability to multiple solution recycling.

Original languageEnglish (US)
Pages (from-to)447-454
Number of pages8
JournalNature Chemistry
Volume7
Issue number5
DOIs
StatePublished - May 1 2015

Fingerprint

Hydrogel
Biomolecules
Sorting
Hydrogels
Thrombin
Binding sites
Hybrid systems
System stability
Microfluidics
Recycling
Binding Sites
Molecules
Fluids
Environmental analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Shastri, A., McGregor, L. M., Liu, Y., Harris, V., Nan, H., Mujica, M., ... He, X. (2015). An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system. Nature Chemistry, 7(5), 447-454. https://doi.org/10.1038/nchem.2203

An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system. / Shastri, Ankita; McGregor, Lynn M.; Liu, Ya; Harris, Valerie; Nan, Hanqing; Mujica, Maritza; Vasquez, Yolanda; Bhattacharya, Amitabh; Ma, Yongting; Aizenberg, Michael; Kuksenok, Olga; Balazs, Anna C.; Aizenberg, Joanna; He, Ximin.

In: Nature Chemistry, Vol. 7, No. 5, 01.05.2015, p. 447-454.

Research output: Contribution to journalArticle

Shastri, A, McGregor, LM, Liu, Y, Harris, V, Nan, H, Mujica, M, Vasquez, Y, Bhattacharya, A, Ma, Y, Aizenberg, M, Kuksenok, O, Balazs, AC, Aizenberg, J & He, X 2015, 'An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system', Nature Chemistry, vol. 7, no. 5, pp. 447-454. https://doi.org/10.1038/nchem.2203
Shastri, Ankita ; McGregor, Lynn M. ; Liu, Ya ; Harris, Valerie ; Nan, Hanqing ; Mujica, Maritza ; Vasquez, Yolanda ; Bhattacharya, Amitabh ; Ma, Yongting ; Aizenberg, Michael ; Kuksenok, Olga ; Balazs, Anna C. ; Aizenberg, Joanna ; He, Ximin. / An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system. In: Nature Chemistry. 2015 ; Vol. 7, No. 5. pp. 447-454.
@article{5a6a27c55166406c9e75aa468e5eed3d,
title = "An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system",
abstract = "The efficient extraction of (bio)molecules from fluid mixtures is vital for applications ranging from target characterization in (bio)chemistry to environmental analysis and biomedical diagnostics. Inspired by biological processes that seamlessly synchronize the capture, transport and release of biomolecules, we designed a robust chemomechanical sorting system capable of the concerted catch and release of target biomolecules from a solution mixture. The hybrid system is composed of target-specific, reversible binding sites attached to microscopic fins embedded in a responsive hydrogel that moves the cargo between two chemically distinct environments. To demonstrate the utility of the system, we focus on the effective separation of thrombin by synchronizing the pH-dependent binding strength of a thrombin-specific aptamer with volume changes of the pH-responsive hydrogel in a biphasic microfluidic regime, and show a non-destructive separation that has a quantitative sorting efficiency, as well as the system's stability and amenability to multiple solution recycling.",
author = "Ankita Shastri and McGregor, {Lynn M.} and Ya Liu and Valerie Harris and Hanqing Nan and Maritza Mujica and Yolanda Vasquez and Amitabh Bhattacharya and Yongting Ma and Michael Aizenberg and Olga Kuksenok and Balazs, {Anna C.} and Joanna Aizenberg and Ximin He",
year = "2015",
month = "5",
day = "1",
doi = "10.1038/nchem.2203",
language = "English (US)",
volume = "7",
pages = "447--454",
journal = "Nature Chemistry",
issn = "1755-4330",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system

AU - Shastri, Ankita

AU - McGregor, Lynn M.

AU - Liu, Ya

AU - Harris, Valerie

AU - Nan, Hanqing

AU - Mujica, Maritza

AU - Vasquez, Yolanda

AU - Bhattacharya, Amitabh

AU - Ma, Yongting

AU - Aizenberg, Michael

AU - Kuksenok, Olga

AU - Balazs, Anna C.

AU - Aizenberg, Joanna

AU - He, Ximin

PY - 2015/5/1

Y1 - 2015/5/1

N2 - The efficient extraction of (bio)molecules from fluid mixtures is vital for applications ranging from target characterization in (bio)chemistry to environmental analysis and biomedical diagnostics. Inspired by biological processes that seamlessly synchronize the capture, transport and release of biomolecules, we designed a robust chemomechanical sorting system capable of the concerted catch and release of target biomolecules from a solution mixture. The hybrid system is composed of target-specific, reversible binding sites attached to microscopic fins embedded in a responsive hydrogel that moves the cargo between two chemically distinct environments. To demonstrate the utility of the system, we focus on the effective separation of thrombin by synchronizing the pH-dependent binding strength of a thrombin-specific aptamer with volume changes of the pH-responsive hydrogel in a biphasic microfluidic regime, and show a non-destructive separation that has a quantitative sorting efficiency, as well as the system's stability and amenability to multiple solution recycling.

AB - The efficient extraction of (bio)molecules from fluid mixtures is vital for applications ranging from target characterization in (bio)chemistry to environmental analysis and biomedical diagnostics. Inspired by biological processes that seamlessly synchronize the capture, transport and release of biomolecules, we designed a robust chemomechanical sorting system capable of the concerted catch and release of target biomolecules from a solution mixture. The hybrid system is composed of target-specific, reversible binding sites attached to microscopic fins embedded in a responsive hydrogel that moves the cargo between two chemically distinct environments. To demonstrate the utility of the system, we focus on the effective separation of thrombin by synchronizing the pH-dependent binding strength of a thrombin-specific aptamer with volume changes of the pH-responsive hydrogel in a biphasic microfluidic regime, and show a non-destructive separation that has a quantitative sorting efficiency, as well as the system's stability and amenability to multiple solution recycling.

UR - http://www.scopus.com/inward/record.url?scp=84928668368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928668368&partnerID=8YFLogxK

U2 - 10.1038/nchem.2203

DO - 10.1038/nchem.2203

M3 - Article

VL - 7

SP - 447

EP - 454

JO - Nature Chemistry

JF - Nature Chemistry

SN - 1755-4330

IS - 5

ER -