An alternative framework for fluorescence correlation spectroscopy

Sina Jazani, Ioannis Sgouralis, Omer M. Shafraz, Marcia Levitus, Sanjeevi Sivasankar, Steve Presse

Research output: Contribution to journalArticle

Abstract

Fluorescence correlation spectroscopy (FCS), is a widely used tool routinely exploited for in vivo and in vitro applications. While FCS provides estimates of dynamical quantities, such as diffusion coefficients, it demands high signal to noise ratios and long time traces, typically in the minute range. In principle, the same information can be extracted from microseconds to seconds long time traces; however, an appropriate analysis method is missing. To overcome these limitations, we adapt novel tools inspired by Bayesian non-parametrics, which starts from the direct analysis of the observed photon counts. With this approach, we are able to analyze time traces, which are too short to be analyzed by existing methods, including FCS. Our new analysis extends the capability of single molecule fluorescence confocal microscopy approaches to probe processes several orders of magnitude faster and permits a reduction of photo-toxic effects on living samples induced by long periods of light exposure.

Original languageEnglish (US)
Article number3662
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

Fluorescence Spectrometry
Fluorescence
Spectroscopy
fluorescence
spectroscopy
Confocal microscopy
Poisons
Fluorescence microscopy
Signal-To-Noise Ratio
Photons
Fluorescence Microscopy
Confocal Microscopy
Signal to noise ratio
Light
Molecules
signal to noise ratios
diffusion coefficient
microscopy
probes
photons

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

An alternative framework for fluorescence correlation spectroscopy. / Jazani, Sina; Sgouralis, Ioannis; Shafraz, Omer M.; Levitus, Marcia; Sivasankar, Sanjeevi; Presse, Steve.

In: Nature communications, Vol. 10, No. 1, 3662, 01.12.2019.

Research output: Contribution to journalArticle

Jazani, Sina ; Sgouralis, Ioannis ; Shafraz, Omer M. ; Levitus, Marcia ; Sivasankar, Sanjeevi ; Presse, Steve. / An alternative framework for fluorescence correlation spectroscopy. In: Nature communications. 2019 ; Vol. 10, No. 1.
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