An altered rpoS allele contributes to the avirulence of Salmonella typhimurium LT2

Mary R. Wilmes-Riesenberg, John W. Foster, Roy Curtiss

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Virulent Salmonella typhimurium strains differ from the attenuated laboratory strain LT2 at the rpoS locus. It was previously shown that the rpoS gene in strain LT2 contains a rare UUG start codon (I. S. Lee, J. Lin, H. K. Hall, B. Bearson, and J. W. Foster, Mol. Microbiol. 17:155-167, 1995). This difference is responsible for the inability of LT2 to display a sustained log-phase acid tolerance response. We show that the altered rpoS allele (rpoS(LT2)) also affects the stationary-phase acid tolerance response in Salmonella. By transducing the rpoS(LT2) allele into virulent strain backgrounds and crossing wild-type rpoS allele into strain LT2, we demonstrate that the rpoS(LT2) allele contributes to the attenuation of strain LT2. We examined the effect of the rpoS allele on invasion and found that the rpoS status of the cell had no effect on the ability of the strains to invade intestinal epithelial cells in tissue culture. Enumeration of bacteria from tissues of infected mice indicated that the presence of the rpoS(LT2) allele affected the ability of S. typhimurium to reach the liver and spleen and to persist in several tissues at 6 days postinfection. This is likely due, at least in part, to a decrease in spv gene expression in these mutants. We demonstrate that strains containing the rpoS(LT2) allele are not only sensitive to pH 3.0 (acid stress) but are also sensitive to the DNA- damaging agent methyl methanesulfonate. However, these strains appear to survive stationary-phase and oxidative stresses as well as strains containing a wild-type rpoS allele. Despite an increased sensitivity to acid stress and DNA damage, strains containing either an rpoS-null mutation or the rpoS(LT2) allele survived in J774 cells and bone marrow-derived macrophages as well as did otherwise isogenic strains with a wild-type rpoS allele.

Original languageEnglish (US)
Pages (from-to)203-210
Number of pages8
JournalInfection and Immunity
Volume65
Issue number1
StatePublished - Jan 1997
Externally publishedYes

Fingerprint

Salmonella typhimurium
Alleles
Acids
Methyl Methanesulfonate
Initiator Codon
Salmonella
DNA Damage
Oxidative Stress
Spleen
Epithelial Cells
Macrophages
Bacteria
Gene Expression
Mutation
Liver
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Wilmes-Riesenberg, M. R., Foster, J. W., & Curtiss, R. (1997). An altered rpoS allele contributes to the avirulence of Salmonella typhimurium LT2. Infection and Immunity, 65(1), 203-210.

An altered rpoS allele contributes to the avirulence of Salmonella typhimurium LT2. / Wilmes-Riesenberg, Mary R.; Foster, John W.; Curtiss, Roy.

In: Infection and Immunity, Vol. 65, No. 1, 01.1997, p. 203-210.

Research output: Contribution to journalArticle

Wilmes-Riesenberg, MR, Foster, JW & Curtiss, R 1997, 'An altered rpoS allele contributes to the avirulence of Salmonella typhimurium LT2', Infection and Immunity, vol. 65, no. 1, pp. 203-210.
Wilmes-Riesenberg MR, Foster JW, Curtiss R. An altered rpoS allele contributes to the avirulence of Salmonella typhimurium LT2. Infection and Immunity. 1997 Jan;65(1):203-210.
Wilmes-Riesenberg, Mary R. ; Foster, John W. ; Curtiss, Roy. / An altered rpoS allele contributes to the avirulence of Salmonella typhimurium LT2. In: Infection and Immunity. 1997 ; Vol. 65, No. 1. pp. 203-210.
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