An acid-tolerance response system protecting exponentially growing Escherichia coli

Ying Xu, Zhe Zhao, Wenhua Tong, Yamei Ding, Bin Liu, Yixin Shi, Jichao Wang, Shenmei Sun, Min Liu, Yuhui Wang, Qingsheng Qi, Mo Xian, Guang Zhao

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The ability to grow at moderate acidic conditions (pH 4.0–5.0) is important to Escherichia coli colonization of the host’s intestine. Several regulatory systems are known to control acid resistance in E. coli, enabling the bacteria to survive under acidic conditions without growth. Here, we characterize an acid-tolerance response (ATR) system and its regulatory circuit, required for E. coli exponential growth at pH 4.2. A two-component system CpxRA directly senses acidification through protonation of CpxA periplasmic histidine residues, and upregulates the fabA and fabB genes, leading to increased production of unsaturated fatty acids. Changes in lipid composition decrease membrane fluidity, F0F1-ATPase activity, and improve intracellular pH homeostasis. The ATR system is important for E. coli survival in the mouse intestine and for production of higher level of 3-hydroxypropionate during fermentation. Furthermore, this ATR system appears to be conserved in other Gram-negative bacteria.

Original languageEnglish (US)
Article number1496
JournalNature communications
Volume11
Issue number1
DOIs
StatePublished - Dec 1 2020

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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  • Cite this

    Xu, Y., Zhao, Z., Tong, W., Ding, Y., Liu, B., Shi, Y., Wang, J., Sun, S., Liu, M., Wang, Y., Qi, Q., Xian, M., & Zhao, G. (2020). An acid-tolerance response system protecting exponentially growing Escherichia coli. Nature communications, 11(1), [1496]. https://doi.org/10.1038/s41467-020-15350-5