Amyloid β analysis from microdissected brain cells using microfluidics and maldi mass spectrometry

Jorvani Cruz Villarreal, Ana Egatz-Gomez, Jiawei Liu, Robert Ros, Paul D. Coleman, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Soluble amyloid β (Aβ) oligomers have been identified as the main species responsible for neuronal dysfunction in Alzheimer's Disease (AD), but details on their chemical nature are still not unraveled. We are developing a microfluidic assay to analyze Aβ species from microdissected human brain cells with MALDI mass spectrometry (MS) for chemical identification. Here, we report the capture of microdissected brain cells into microfluidic platforms, Aβ immunocapture with suitable antibodies, and Aβ42 species characterization using MALDI-MS and atomic force microscopy. This approach will allow to investigate soluble Aβ species in AD post-mortem brains providing insights into their role in AD.

Original languageEnglish (US)
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages711-712
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Keywords

  • Amyloid-beta
  • Laser-microdissection
  • Mass Spectrometry
  • Microfluidics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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