Amperometric sensing of norepinephrine at picomolar concentrations using screen printed, high surface area mesoporous carbon

Mingzhi Dai, Brittney Haselwood, Bryan D. Vogt, Jeffrey LaBelle

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Norepinephrine (NE) is detected amperometrically using the enzyme Phenylethanolamine N-methyl transferase and cofactor S-(5'-Adenosyl)-l-methionine chloride dihydrochloride with disposable screen printed mesoporous carbon electrodes. The role of internal surface area and pore size of the mesoporous carbon is systematically examined using soft-templated, mesoporous silica-carbon powders with highly microporous walls obtained from etching of the silica to produce powders with surface areas ranging from 671-2339m2g-1. As the surface area increases, the sensitivity of the biosensor at very low NE concentrations (0-500pgmL-1) in phosphate buffered saline (PBS) increases just as the current signal increases with respect to the NE concentration of 81-1581μAmLng-1cm-2 for the mesoporous carbons. The best performing electrode provides similar sensitivity in whole rabbit blood in comparison to PBS despite no membrane layer to filter the non-desired reactants; the small (<5nm) pore size and large internal surface area acts to minimize non-specific events that decrease sensitivity.

Original languageEnglish (US)
Pages (from-to)32-38
Number of pages7
JournalAnalytica Chimica Acta
Volume788
DOIs
StatePublished - Jul 25 2013

Keywords

  • Electrochemistry
  • Mesoporous carbon
  • Norepinephrine
  • Porous materials
  • Self-assembly
  • Sensor

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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