Amperometric determination of dopamine on an enzymatically modified carbon paste electrode

E. S. Forzani; G. A. Rivas; V. M. Solis

doi:10.1016/0022-0728(94)03640-O

Amperometric determination of dopamine on an enzymatically modified carbon paste electrode

E. S. Forzani, G. A. Rivas, V. M. Solis

Research output: Contribution to journal › Article › peer-review

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Abstract

The amperometric determination of dopamine under controlled convective conditions on an enzymatically modified carbon paste electrode was studied using potato tissue as a source of polyphenol oxidase. Enzymatic kinetic constants and substrate diffusional-resistance parameters were determined and an analysis of the most common interferents was made. It is concluded that the electrode is suitable for the quantification of dopamine with a detection limit as low as 2.5 × 10^-6 M when the hydrodynamic conditions are carefully controlled. The electrode is active towards dopac, ascorbic acid and pyrocatechol but not l-tyrosine, l-dopa, homovanillic acid, adrenaline, normetanephrine and vanilmandelic acid.

Original language	English (US)
Pages (from-to)	33-40
Number of pages	8
Journal	Journal of Electroanalytical Chemistry
Volume	382
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-0728(94)03640-O
State	Published - Feb 7 1995
Externally published	Yes

Keywords

Amperometry
Carbon paste electrode
Dopamine
Enzyme electrode

ASJC Scopus subject areas

Analytical Chemistry
General Chemical Engineering
Electrochemistry

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10.1016/0022-0728(94)03640-O

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title = "Amperometric determination of dopamine on an enzymatically modified carbon paste electrode",

abstract = "The amperometric determination of dopamine under controlled convective conditions on an enzymatically modified carbon paste electrode was studied using potato tissue as a source of polyphenol oxidase. Enzymatic kinetic constants and substrate diffusional-resistance parameters were determined and an analysis of the most common interferents was made. It is concluded that the electrode is suitable for the quantification of dopamine with a detection limit as low as 2.5 × 10-6 M when the hydrodynamic conditions are carefully controlled. The electrode is active towards dopac, ascorbic acid and pyrocatechol but not l-tyrosine, l-dopa, homovanillic acid, adrenaline, normetanephrine and vanilmandelic acid.",

keywords = "Amperometry, Carbon paste electrode, Dopamine, Enzyme electrode",

author = "Forzani, {E. S.} and Rivas, {G. A.} and Solis, {V. M.}",

note = "Funding Information: Financial support from the Consejo Nacional de InvestigacioneCsie ntificasy Tecnol6gica(sC ONICET) and Consejo de Investigacionedse la Provincia de C6rdoba( CONICOR) is gratefullya cknowledged. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.",

year = "1995",

month = feb,

day = "7",

doi = "10.1016/0022-0728(94)03640-O",

language = "English (US)",

volume = "382",

pages = "33--40",

journal = "Journal of Electroanalytical Chemistry",

issn = "0022-0728",

publisher = "Elsevier Sequoia",

number = "1-2",

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TY - JOUR

T1 - Amperometric determination of dopamine on an enzymatically modified carbon paste electrode

AU - Forzani, E. S.

AU - Rivas, G. A.

AU - Solis, V. M.

N1 - Funding Information: Financial support from the Consejo Nacional de InvestigacioneCsie ntificasy Tecnol6gica(sC ONICET) and Consejo de Investigacionedse la Provincia de C6rdoba( CONICOR) is gratefullya cknowledged. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.

PY - 1995/2/7

Y1 - 1995/2/7

N2 - The amperometric determination of dopamine under controlled convective conditions on an enzymatically modified carbon paste electrode was studied using potato tissue as a source of polyphenol oxidase. Enzymatic kinetic constants and substrate diffusional-resistance parameters were determined and an analysis of the most common interferents was made. It is concluded that the electrode is suitable for the quantification of dopamine with a detection limit as low as 2.5 × 10-6 M when the hydrodynamic conditions are carefully controlled. The electrode is active towards dopac, ascorbic acid and pyrocatechol but not l-tyrosine, l-dopa, homovanillic acid, adrenaline, normetanephrine and vanilmandelic acid.

AB - The amperometric determination of dopamine under controlled convective conditions on an enzymatically modified carbon paste electrode was studied using potato tissue as a source of polyphenol oxidase. Enzymatic kinetic constants and substrate diffusional-resistance parameters were determined and an analysis of the most common interferents was made. It is concluded that the electrode is suitable for the quantification of dopamine with a detection limit as low as 2.5 × 10-6 M when the hydrodynamic conditions are carefully controlled. The electrode is active towards dopac, ascorbic acid and pyrocatechol but not l-tyrosine, l-dopa, homovanillic acid, adrenaline, normetanephrine and vanilmandelic acid.

KW - Amperometry

KW - Carbon paste electrode

KW - Dopamine

KW - Enzyme electrode

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U2 - 10.1016/0022-0728(94)03640-O

DO - 10.1016/0022-0728(94)03640-O

M3 - Article

AN - SCOPUS:0001818922

SN - 0022-0728

VL - 382

SP - 33

EP - 40

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

IS - 1-2

ER -

Amperometric determination of dopamine on an enzymatically modified carbon paste electrode

Abstract

Keywords

ASJC Scopus subject areas

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