Amperometric determination of dopamine on an enzymatically modified carbon paste electrode

E. S. Forzani, G. A. Rivas, V. M. Solis

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

The amperometric determination of dopamine under controlled convective conditions on an enzymatically modified carbon paste electrode was studied using potato tissue as a source of polyphenol oxidase. Enzymatic kinetic constants and substrate diffusional-resistance parameters were determined and an analysis of the most common interferents was made. It is concluded that the electrode is suitable for the quantification of dopamine with a detection limit as low as 2.5 × 10-6 M when the hydrodynamic conditions are carefully controlled. The electrode is active towards dopac, ascorbic acid and pyrocatechol but not l-tyrosine, l-dopa, homovanillic acid, adrenaline, normetanephrine and vanilmandelic acid.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalJournal of Electroanalytical Chemistry
Volume382
Issue number1-2
DOIs
StatePublished - Feb 7 1995
Externally publishedYes

Keywords

  • Amperometry
  • Carbon paste electrode
  • Dopamine
  • Enzyme electrode

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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