Ammonia-oxidizing archaea and bacteria are structured by geography in biological soil crusts across North American arid lands

Yevgeniy Marusenko, Scott T. Bates, Ian Anderson, Shannon L. Johnson, Tanya Soule, Ferran Garcia-Pichel

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Introduction: Biological soil crusts (BSCs) can dominate surface cover in dry lands worldwide, playing an integral role in arid land biogeochemistry, particularly in N fertilization through fixation and cycling. Nitrification is a characteristic and universal N transformation in BSCs that becomes important for the export of N beyond the microscopic bounds of the crust itself. The contribution of ammonia-oxidizing bacteria (AOB) in BSCs has been shown, but the role and extent of the recently discovered ammonia-oxidizing archaea (AOA) have not. Methods: We sampled various types of crusts in four desert regions across the western United States and characterized the composition and size of ammonia-oxidizing communities using clone libraries and quantitative PCR targeting the amoA gene, which codes for the ammonia monooxygenase enzyme, universally present in ammonia-oxidizing microbes. Results: All archaeal amoA sequences retrieved from BSCs belonged to the Thaumarchaeota (Nitrososphaera associated Group I.1b). Sequences from the Sonoran Desert, Colorado Plateau, and Great Basin were indistinguishable from each other but distinct from those of the Chihuahuan Desert. Based on amoA gene abundances, archaeal and bacterial ammonia oxidizers were ubiquitous in our survey, but the ratios of archaeal to bacterial ammonia oxidizers shifted from bacterially dominated in northern, cooler deserts to archaeally dominated in southern, warmer deserts. Conclusions: Archaea are shown to be potentially important biogeochemical agents of biological soil crust N cycling. Conditions associated with different types of BSCs and biogeographical factors reveal a niche differentiation between AOA and AOB, possibly driven by temperature.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalEcological Processes
Volume2
Issue number1
DOIs
StatePublished - 2013

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soil crust
ammonia
bacterium
desert
crust
geography
arid land
gene
biogeochemistry
targeting
fixation
nitrification
clone
niche
plateau
enzyme

Keywords

  • Ammonia oxidation
  • amoA
  • Arid
  • Biological soil crust
  • Desert
  • Nitrification
  • Nitrogen cycle
  • Thaumarchaeota

ASJC Scopus subject areas

  • Ecological Modeling
  • Ecology

Cite this

Ammonia-oxidizing archaea and bacteria are structured by geography in biological soil crusts across North American arid lands. / Marusenko, Yevgeniy; Bates, Scott T.; Anderson, Ian; Johnson, Shannon L.; Soule, Tanya; Garcia-Pichel, Ferran.

In: Ecological Processes, Vol. 2, No. 1, 2013, p. 1-10.

Research output: Contribution to journalArticle

Marusenko, Yevgeniy ; Bates, Scott T. ; Anderson, Ian ; Johnson, Shannon L. ; Soule, Tanya ; Garcia-Pichel, Ferran. / Ammonia-oxidizing archaea and bacteria are structured by geography in biological soil crusts across North American arid lands. In: Ecological Processes. 2013 ; Vol. 2, No. 1. pp. 1-10.
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AB - Introduction: Biological soil crusts (BSCs) can dominate surface cover in dry lands worldwide, playing an integral role in arid land biogeochemistry, particularly in N fertilization through fixation and cycling. Nitrification is a characteristic and universal N transformation in BSCs that becomes important for the export of N beyond the microscopic bounds of the crust itself. The contribution of ammonia-oxidizing bacteria (AOB) in BSCs has been shown, but the role and extent of the recently discovered ammonia-oxidizing archaea (AOA) have not. Methods: We sampled various types of crusts in four desert regions across the western United States and characterized the composition and size of ammonia-oxidizing communities using clone libraries and quantitative PCR targeting the amoA gene, which codes for the ammonia monooxygenase enzyme, universally present in ammonia-oxidizing microbes. Results: All archaeal amoA sequences retrieved from BSCs belonged to the Thaumarchaeota (Nitrososphaera associated Group I.1b). Sequences from the Sonoran Desert, Colorado Plateau, and Great Basin were indistinguishable from each other but distinct from those of the Chihuahuan Desert. Based on amoA gene abundances, archaeal and bacterial ammonia oxidizers were ubiquitous in our survey, but the ratios of archaeal to bacterial ammonia oxidizers shifted from bacterially dominated in northern, cooler deserts to archaeally dominated in southern, warmer deserts. Conclusions: Archaea are shown to be potentially important biogeochemical agents of biological soil crust N cycling. Conditions associated with different types of BSCs and biogeographical factors reveal a niche differentiation between AOA and AOB, possibly driven by temperature.

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