Alterations in Hippocampal Network Activity after In Vitro Traumatic Brain Injury

Woo Hyeun Kang, Wenzhe Cao, Oliver Graudejus, Tapan P. Patel, Sigurd Wagner, David F. Meaney, Barclay Morrison

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Traumatic brain injury (TBI) alters function and behavior, which can be characterized by changes in electrophysiological function in vitro. A common cognitive deficit after mild-to-moderate TBI is disruption of persistent working memory, of which the in vitro correlate is long-lasting, neuronal network synchronization that can be induced pharmacologically by the gamma-aminobutyric acid A antagonist, bicuculline. We utilized a novel in vitro platform for TBI research, the stretchable microelectrode array (SMEA), to investigate the effects of TBI on bicuculline-induced, long-lasting network synchronization in the hippocampus. Mechanical stimulation significantly disrupted bicuculline-induced, long-lasting network synchronization 24 h after injury, despite the continued ability of the injured neurons to fire, as revealed by a significant increase in the normalized spontaneous event rate in the dentate gyrus (DG) and CA1. A second challenge with bicuculline 24 h after the first challenge significantly decreased the normalized spontaneous event rate in the DG. In addition, we illustrate the utility of the SMEA for TBI research by combining multiple experimental paradigms in one platform, which has the potential to enable novel investigations into the mechanisms responsible for functional consequences of TBI and speed the rate of drug discovery.

Original languageEnglish (US)
Pages (from-to)1011-1019
Number of pages9
JournalJournal of Neurotrauma
Volume32
Issue number13
DOIs
StatePublished - Jan 1 2015

Fingerprint

Bicuculline
Dentate Gyrus
Microelectrodes
GABA Antagonists
Aptitude
Drug Discovery
Short-Term Memory
Research
In Vitro Techniques
Traumatic Brain Injury
Hippocampus
Neurons
Wounds and Injuries

Keywords

  • electrophysiology
  • hippocampus
  • network synchronization
  • traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Kang, W. H., Cao, W., Graudejus, O., Patel, T. P., Wagner, S., Meaney, D. F., & Morrison, B. (2015). Alterations in Hippocampal Network Activity after In Vitro Traumatic Brain Injury. Journal of Neurotrauma, 32(13), 1011-1019. https://doi.org/10.1089/neu.2014.3667

Alterations in Hippocampal Network Activity after In Vitro Traumatic Brain Injury. / Kang, Woo Hyeun; Cao, Wenzhe; Graudejus, Oliver; Patel, Tapan P.; Wagner, Sigurd; Meaney, David F.; Morrison, Barclay.

In: Journal of Neurotrauma, Vol. 32, No. 13, 01.01.2015, p. 1011-1019.

Research output: Contribution to journalArticle

Kang, WH, Cao, W, Graudejus, O, Patel, TP, Wagner, S, Meaney, DF & Morrison, B 2015, 'Alterations in Hippocampal Network Activity after In Vitro Traumatic Brain Injury', Journal of Neurotrauma, vol. 32, no. 13, pp. 1011-1019. https://doi.org/10.1089/neu.2014.3667
Kang, Woo Hyeun ; Cao, Wenzhe ; Graudejus, Oliver ; Patel, Tapan P. ; Wagner, Sigurd ; Meaney, David F. ; Morrison, Barclay. / Alterations in Hippocampal Network Activity after In Vitro Traumatic Brain Injury. In: Journal of Neurotrauma. 2015 ; Vol. 32, No. 13. pp. 1011-1019.
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