Alterations in a yeast protein resembling HIV Tat-binding protein relieve requirement for an acidic activation domain in GAL4

Jonathan C. Swaffield, Jacqueline F. Bromberg, Stephen A. Johnston

Research output: Contribution to journalArticle

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Abstract

THE acidic transcriptional activation motif functions in all eukary-otes1-4, which suggests that it makes contact with some universal component of the transcriptional apparatus. Transcriptional activation by the yeast regulatory protein GAL4 requires an acidic region at its carboxyl terminus. Here we implement a selection scheme to determine whether GAL4 can still function when this C-terminal domain has been deleted. It can, when accompanied by a mutation in the SUG1gene which is an essential gene in yeast. Analysis of mutant SUG1in combination with various alleles of GAL4 indicates that SUG1 acts through a transcriptional pathway that depends on GAL4, but requires a region of GAL4 other than the C-terminal acidic activation domain. The predicted amino-acid sequence of SUG1 closely resembles that of two human proteins, TBP1 and MSS1, which modulate expression mediated by the human immunodeficiency virus tatgene.

Original languageEnglish (US)
Pages (from-to)698-700
Number of pages3
JournalNature
Volume357
Issue number6380
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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