Alterations in a yeast protein resembling HIV Tat-binding protein relieve requirement for an acidic activation domain in GAL4

Jonathan C. Swaffield, Jacqueline F. Bromberg, Stephen Johnston

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

The acidic transcriptional activation motif functions in all eukaryotes1-4, which suggests that it makes contact with some universal component of the transcriptional apparatus. Transcriptional activation by the yeast regulatory protein GAL4 requires an acidic region at its carboxyl terminus. Here we implement a selection scheme to determine whether GAL4 can still function when this C-terminal domain has been deleted. It can, when accompanied by a mutation in the SUG1 gene which is an essential gene in yeast. Analysis of mutant SUG1 in combination with various alleles of GALA indicates that SUG1 acts through a transcriptional pathway that depends on GAL4, but requires a region of GAL4 other than the C-terminal acidic activation domain. The predicted amino-acid sequence of SUG1 closely resembles that of two human proteins, TBP1 and MSS1, which modulate expression mediated by the human immunodeficiency virus tat gene.

Original languageEnglish (US)
Pages (from-to)698-700
Number of pages3
JournalNature
Volume357
Issue number6380
StatePublished - Jun 25 1992
Externally publishedYes

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Human Immunodeficiency Virus tat Gene Products
Fungal Proteins
Transcriptional Activation
Carrier Proteins
tat Genes
Essential Genes
Amino Acid Sequence
Yeasts
Alleles
HIV
Mutation
Genes

ASJC Scopus subject areas

  • General

Cite this

Alterations in a yeast protein resembling HIV Tat-binding protein relieve requirement for an acidic activation domain in GAL4. / Swaffield, Jonathan C.; Bromberg, Jacqueline F.; Johnston, Stephen.

In: Nature, Vol. 357, No. 6380, 25.06.1992, p. 698-700.

Research output: Contribution to journalArticle

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