Algal biomass constituent analysis: Method uncertainties and investigation of the underlying measuring chemistries

Lieve M L Laurens, Thomas Dempster, Howland D T Jones, Edward J. Wolfrum, Stefanie Van Wychen, Jordan S P McAllister, Michelle Rencenberger, Kylea J. Parchert, Lindsey M. Gloe

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Algal biomass compositional analysis data form the basis of a large number of techno-economic process analysis models that are used to investigate and compare different processes in algal biofuels production. However, the analytical methods used to generate these data are far from standardized. This work investigated the applicability of common methods for rapid chemical analysis of biomass samples with respect to accuracy and precision. This study measured lipids, protein, carbohydrates, ash, and moisture of a single algal biomass sample at 3 institutions by 8 independent researchers over 12 separate workdays. Results show statistically significant differences in the results from a given analytical method among laboratories but not between analysts at individual laboratories, suggesting consistent training is a critical issue for empirical analytical methods. Significantly different results from multiple lipid and protein measurements were found to be due to different measurement chemistries. We identified a set of compositional analysis procedures that are in best agreement with data obtained by more advanced analytical procedures. The methods described here and used for the round robin experiment do not require specialized instrumentation, and with detailed analytical documentation, the differences between laboratories can be markedly reduced.

Original languageEnglish (US)
Pages (from-to)1879-1887
Number of pages9
JournalAnalytical Chemistry
Volume84
Issue number4
DOIs
StatePublished - Feb 21 2012

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Biomass
Ashes
Lipids
Biofuels
Proteins
Moisture
Carbohydrates
Economics
Chemical analysis
Uncertainty
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Laurens, L. M. L., Dempster, T., Jones, H. D. T., Wolfrum, E. J., Van Wychen, S., McAllister, J. S. P., ... Gloe, L. M. (2012). Algal biomass constituent analysis: Method uncertainties and investigation of the underlying measuring chemistries. Analytical Chemistry, 84(4), 1879-1887. https://doi.org/10.1021/ac202668c

Algal biomass constituent analysis : Method uncertainties and investigation of the underlying measuring chemistries. / Laurens, Lieve M L; Dempster, Thomas; Jones, Howland D T; Wolfrum, Edward J.; Van Wychen, Stefanie; McAllister, Jordan S P; Rencenberger, Michelle; Parchert, Kylea J.; Gloe, Lindsey M.

In: Analytical Chemistry, Vol. 84, No. 4, 21.02.2012, p. 1879-1887.

Research output: Contribution to journalArticle

Laurens, LML, Dempster, T, Jones, HDT, Wolfrum, EJ, Van Wychen, S, McAllister, JSP, Rencenberger, M, Parchert, KJ & Gloe, LM 2012, 'Algal biomass constituent analysis: Method uncertainties and investigation of the underlying measuring chemistries', Analytical Chemistry, vol. 84, no. 4, pp. 1879-1887. https://doi.org/10.1021/ac202668c
Laurens LML, Dempster T, Jones HDT, Wolfrum EJ, Van Wychen S, McAllister JSP et al. Algal biomass constituent analysis: Method uncertainties and investigation of the underlying measuring chemistries. Analytical Chemistry. 2012 Feb 21;84(4):1879-1887. https://doi.org/10.1021/ac202668c
Laurens, Lieve M L ; Dempster, Thomas ; Jones, Howland D T ; Wolfrum, Edward J. ; Van Wychen, Stefanie ; McAllister, Jordan S P ; Rencenberger, Michelle ; Parchert, Kylea J. ; Gloe, Lindsey M. / Algal biomass constituent analysis : Method uncertainties and investigation of the underlying measuring chemistries. In: Analytical Chemistry. 2012 ; Vol. 84, No. 4. pp. 1879-1887.
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