Algal biomass constituent analysis: Method uncertainties and investigation of the underlying measuring chemistries

Lieve M L Laurens, Thomas Dempster, Howland D T Jones, Edward J. Wolfrum, Stefanie Van Wychen, Jordan S P McAllister, Michelle Rencenberger, Kylea J. Parchert, Lindsey M. Gloe

    Research output: Contribution to journalArticle

    88 Citations (Scopus)

    Abstract

    Algal biomass compositional analysis data form the basis of a large number of techno-economic process analysis models that are used to investigate and compare different processes in algal biofuels production. However, the analytical methods used to generate these data are far from standardized. This work investigated the applicability of common methods for rapid chemical analysis of biomass samples with respect to accuracy and precision. This study measured lipids, protein, carbohydrates, ash, and moisture of a single algal biomass sample at 3 institutions by 8 independent researchers over 12 separate workdays. Results show statistically significant differences in the results from a given analytical method among laboratories but not between analysts at individual laboratories, suggesting consistent training is a critical issue for empirical analytical methods. Significantly different results from multiple lipid and protein measurements were found to be due to different measurement chemistries. We identified a set of compositional analysis procedures that are in best agreement with data obtained by more advanced analytical procedures. The methods described here and used for the round robin experiment do not require specialized instrumentation, and with detailed analytical documentation, the differences between laboratories can be markedly reduced.

    Original languageEnglish (US)
    Pages (from-to)1879-1887
    Number of pages9
    JournalAnalytical Chemistry
    Volume84
    Issue number4
    DOIs
    StatePublished - Feb 21 2012

    Fingerprint

    Biomass
    Ashes
    Lipids
    Biofuels
    Proteins
    Moisture
    Carbohydrates
    Economics
    Chemical analysis
    Uncertainty
    Experiments

    ASJC Scopus subject areas

    • Analytical Chemistry

    Cite this

    Laurens, L. M. L., Dempster, T., Jones, H. D. T., Wolfrum, E. J., Van Wychen, S., McAllister, J. S. P., ... Gloe, L. M. (2012). Algal biomass constituent analysis: Method uncertainties and investigation of the underlying measuring chemistries. Analytical Chemistry, 84(4), 1879-1887. https://doi.org/10.1021/ac202668c

    Algal biomass constituent analysis : Method uncertainties and investigation of the underlying measuring chemistries. / Laurens, Lieve M L; Dempster, Thomas; Jones, Howland D T; Wolfrum, Edward J.; Van Wychen, Stefanie; McAllister, Jordan S P; Rencenberger, Michelle; Parchert, Kylea J.; Gloe, Lindsey M.

    In: Analytical Chemistry, Vol. 84, No. 4, 21.02.2012, p. 1879-1887.

    Research output: Contribution to journalArticle

    Laurens, LML, Dempster, T, Jones, HDT, Wolfrum, EJ, Van Wychen, S, McAllister, JSP, Rencenberger, M, Parchert, KJ & Gloe, LM 2012, 'Algal biomass constituent analysis: Method uncertainties and investigation of the underlying measuring chemistries' Analytical Chemistry, vol. 84, no. 4, pp. 1879-1887. https://doi.org/10.1021/ac202668c
    Laurens, Lieve M L ; Dempster, Thomas ; Jones, Howland D T ; Wolfrum, Edward J. ; Van Wychen, Stefanie ; McAllister, Jordan S P ; Rencenberger, Michelle ; Parchert, Kylea J. ; Gloe, Lindsey M. / Algal biomass constituent analysis : Method uncertainties and investigation of the underlying measuring chemistries. In: Analytical Chemistry. 2012 ; Vol. 84, No. 4. pp. 1879-1887.
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