ALG-1 Influences Accurate mRNA Splicing Patterns in the Caenorhabditis elegans Intestine and Body Muscle Tissues by Modulating Splicing Factor Activities

Kasuen Kotagama, Anna L. Schorr, Hannah S. Steber, Marco Mangone

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs) are known to modulate gene expression, but their activity at the tissue-specific level remains largely uncharacterized. To study their contribution to tissue-specific gene expression, we developed novel tools to profile putative miRNA targets in the Caenorhabditis elegans intestine and body muscle. We validated many previously described interactions and identified ∼3500 novel targets. Many of the candidate miRNA targets curated are known to modulate the functions of their respective tissues. Within our data sets we observed a disparity in the use of miRNA-based gene regulation between the intestine and body muscle. The intestine contained significantly more putative miRNA targets than the body muscle highlighting its transcriptional complexity. We detected an unexpected enrichment of RNA-binding proteins targeted by miRNA in both tissues, with a notable abundance of RNA splicing factors. We developed in vivo genetic tools to validate and further study three RNA splicing factors identified as putative miRNA targets in our study (asd-2, hrp-2, and smu-2), and show that these factors indeed contain functional miRNA regulatory elements in their 3'UTRs that are able to repress their expression in the intestine. In addition, the alternative splicing pattern of their respective downstream targets (unc-60, unc-52, lin-10, and ret-1) is dysregulated when the miRNA pathway is disrupted. A reannotation of the transcriptome data in C. elegans strains that are deficient in the miRNA pathway from past studies supports and expands on our results. This study highlights an unexpected role for miRNAs in modulating tissue-specific gene isoforms, where post-transcriptional regulation of RNA splicing factors associates with tissue-specific alternative splicing.

Original languageEnglish (US)
Pages (from-to)931-951
Number of pages21
JournalGenetics
Volume212
Issue number3
DOIs
StatePublished - Jul 1 2019

Fingerprint

Caenorhabditis elegans
MicroRNAs
Intestines
Muscles
Messenger RNA
Alternative Splicing
RNA Splicing Factors
Gene Expression
RNA-Binding Proteins
3' Untranslated Regions
Transcriptome
Genes
Protein Isoforms

Keywords

  • ALG-1
  • alternative splicing
  • asd-2
  • body muscle
  • Caenorhabditis elegans
  • hrp-2
  • intestine
  • miRNA
  • smu-2
  • transcriptome

ASJC Scopus subject areas

  • Genetics

Cite this

ALG-1 Influences Accurate mRNA Splicing Patterns in the Caenorhabditis elegans Intestine and Body Muscle Tissues by Modulating Splicing Factor Activities. / Kotagama, Kasuen; Schorr, Anna L.; Steber, Hannah S.; Mangone, Marco.

In: Genetics, Vol. 212, No. 3, 01.07.2019, p. 931-951.

Research output: Contribution to journalArticle

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