AFM imaging of protein movements: Histone H2A-H2B release during nucleosome remodeling

R. Bash; H. Wang; C. Anderson; J. Yodh; G. Hager; Stuart Lindsay; D. Lohr

doi:10.1016/j.febslet.2006.06.101

AFM imaging of protein movements: Histone H2A-H2B release during nucleosome remodeling

R. Bash, H. Wang, C. Anderson, J. Yodh, G. Hager, Stuart Lindsay, D. Lohr

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Being able to follow assembly/disassembly reactions of biomolecular complexes directly at the single molecule level would be very useful. Here, we use an AFM technique that can simultaneously obtain topographic images and identify the locations of a specific type of protein within those images to monitor the histone H2A component of nucleosomes acted on by human Swi-Snf, an ATP-dependent nucleosome remodeling complex. Activation of remodeling results in significant H2A release from nucleosomes, based on recognition imaging and nucleosome height changes, and changes in the recognition patterns of H2A associated directly with hSwi-Snf complexes.

Original language	English (US)
Pages (from-to)	4757-4761
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2006.06.101
State	Published - Aug 21 2006

Keywords

AFM
Chromatin
Histones
Nucleosome
Remodeling
SPM
Swi-Snf

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.06.101

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title = "AFM imaging of protein movements: Histone H2A-H2B release during nucleosome remodeling",

abstract = "Being able to follow assembly/disassembly reactions of biomolecular complexes directly at the single molecule level would be very useful. Here, we use an AFM technique that can simultaneously obtain topographic images and identify the locations of a specific type of protein within those images to monitor the histone H2A component of nucleosomes acted on by human Swi-Snf, an ATP-dependent nucleosome remodeling complex. Activation of remodeling results in significant H2A release from nucleosomes, based on recognition imaging and nucleosome height changes, and changes in the recognition patterns of H2A associated directly with hSwi-Snf complexes.",

keywords = "AFM, Chromatin, Histones, Nucleosome, Remodeling, SPM, Swi-Snf",

author = "R. Bash and H. Wang and C. Anderson and J. Yodh and G. Hager and Stuart Lindsay and D. Lohr",

note = "Funding Information: This work was supported by Grant ROI CA085990-03 from the NIH.",

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doi = "10.1016/j.febslet.2006.06.101",

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T1 - AFM imaging of protein movements

T2 - Histone H2A-H2B release during nucleosome remodeling

AU - Bash, R.

AU - Wang, H.

AU - Anderson, C.

AU - Yodh, J.

AU - Hager, G.

AU - Lindsay, Stuart

AU - Lohr, D.

N1 - Funding Information: This work was supported by Grant ROI CA085990-03 from the NIH.

PY - 2006/8/21

Y1 - 2006/8/21

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AB - Being able to follow assembly/disassembly reactions of biomolecular complexes directly at the single molecule level would be very useful. Here, we use an AFM technique that can simultaneously obtain topographic images and identify the locations of a specific type of protein within those images to monitor the histone H2A component of nucleosomes acted on by human Swi-Snf, an ATP-dependent nucleosome remodeling complex. Activation of remodeling results in significant H2A release from nucleosomes, based on recognition imaging and nucleosome height changes, and changes in the recognition patterns of H2A associated directly with hSwi-Snf complexes.

KW - AFM

KW - Chromatin

KW - Histones

KW - Nucleosome

KW - Remodeling

KW - SPM

KW - Swi-Snf

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AFM imaging of protein movements: Histone H2A-H2B release during nucleosome remodeling

Abstract

Keywords

ASJC Scopus subject areas

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