Adsorption of surfactant lipids by single-walled carbon nanotubes in mouse lung upon pharyngeal aspiration

Alexander A. Kapralov, Wei Hong Feng, Andrew A. Amoscato, Naveena Yanamala, Krishnakumar Balasubramanian, Daniel E. Winnica, Elena R. Kisin, Gregg P. Kotchey, Pingping Gou, Louis J. Sparvero, Prabir Ray, Rama K. Mallampalli, Judith Klein-Seetharaman, Bengt Fadeel, Alexander Star, Anna A. Shvedova, Valerian E. Kagan

Research output: Contribution to journalArticlepeer-review

131 Scopus citations

Abstract

The pulmonary route represents one of the most important portals of entry for nanoparticles into the body. However, the in vivo interactions of nanoparticles with biomolecules of the lung have not been sufficiently studied. Here, using an established mouse model of pharyngeal aspiration of single-walled carbon nanotubes (SWCNTs), we recovered SWCNTs from the bronchoalveolar lavage fluid (BALf), purified them from possible contamination with lung cells, and examined the composition of phospholipids adsorbed on SWCNTs by liquid chromatography mass spectrometry (LC-MS) analysis. We found that SWCNTs selectively adsorbed two types of the most abundant surfactant phospholipids: phosphatidylcholines (PC) and phosphatidylglycerols (PG). Molecular speciation of these phospholipids was also consistent with pulmonary surfactant. Quantitation of adsorbed lipids by LC-MS along with the structural assessments of phospholipid binding by atomic force microscopy and molecular modeling indicated that the phospholipids (∼108 molecules per SWCNT) formed an uninterrupted "coating" whereby the hydrophobic alkyl chains of the phospholipids were adsorbed onto the SWCNT with the polar head groups pointed away from the SWCNT into the aqueous phase. In addition, the presence of surfactant proteins A, B, and D on SWCNTs was determined by LC-MS. Finally, we demonstrated that the presence of this surfactant coating markedly enhanced the in vitro uptake of SWCNTs by macrophages. Taken together, this is the first demonstration of the in vivo adsorption of the surfactant lipids and proteins on SWCNTs in a physiologically relevant animal model.

Original languageEnglish (US)
Pages (from-to)4147-4156
Number of pages10
JournalACS nano
Volume6
Issue number5
DOIs
StatePublished - May 22 2012
Externally publishedYes

Keywords

  • Carbon nanotubes
  • Macrophages
  • Surfactant

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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