Adsorption equilibrium and kinetics of microorganisms on single-wall carbon nanotubes

Shuguang Deng, Venkata K K Upadhyayula, Geoffrey B. Smith, Martha C. Mitchell

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Adsorption equilibrium and kinetics of pure and mixed cultures of Escherichia coli and Staphylococcus aureus on single-walled carbon nanotubes (CNT) aggregates were studied in an effort to develop CNT-based biosensors for quick detection of these bacteria in water. Batch experiments were carried out to measure the adsorption kinetics and equilibrium of pure and mixed culture of E. coli and S. aureus on the CNT aggregates at ambient temperature and various culture concentrations. The CNT aggregates can adsorb significant amounts of E. coli and S. aureus bacteria with different size and shape characteristics. The smaller size S. aureus has a five to ten times faster diffusion rate than E. coli and about 100 times higher adsorption affinity with the carbon nanotube aggregates. Freundlich adsorption model correlates well both the pure component and mixture adsorption equilibrium data. It is quite possible the CNT aggregates have separate adsorption sites for both E. coli and S. aureus. The combined high adsorption affinity and fast adsorption kinetics for S. aureus suggest that even unmodified single-wall carbon nanotubes can selectively differentiate S. aureus and E. coli in water. Transmission electron microscopic analysis qualitatively confirmed the adsorption results and provides direct visualization of the adsorbed bacteria on carbon nanotube aggregates. Both bacteria form biofilms on carbon nanotube aggregates and have a strong tendency to connect with each other rather than with the carbon surface.

Original languageEnglish (US)
Article number4529174
Pages (from-to)954-962
Number of pages9
JournalIEEE Sensors Journal
Volume8
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

Fingerprint

microorganisms
Microorganisms
Carbon nanotubes
carbon nanotubes
Adsorption
Escherichia coli
Kinetics
adsorption
kinetics
bacteria
Bacteria
affinity
biofilms
staphylococcus
Biofilms
Single-walled carbon nanotubes (SWCN)
Escherichia
bioinstrumentation
Biosensors
water

Keywords

  • Adsorption
  • Carbon nanotubes (CNTs)
  • Diffusion
  • E. Coli
  • S. Aureus

ASJC Scopus subject areas

  • Engineering(all)
  • Electrical and Electronic Engineering

Cite this

Adsorption equilibrium and kinetics of microorganisms on single-wall carbon nanotubes. / Deng, Shuguang; Upadhyayula, Venkata K K; Smith, Geoffrey B.; Mitchell, Martha C.

In: IEEE Sensors Journal, Vol. 8, No. 6, 4529174, 06.2008, p. 954-962.

Research output: Contribution to journalArticle

Deng, Shuguang ; Upadhyayula, Venkata K K ; Smith, Geoffrey B. ; Mitchell, Martha C. / Adsorption equilibrium and kinetics of microorganisms on single-wall carbon nanotubes. In: IEEE Sensors Journal. 2008 ; Vol. 8, No. 6. pp. 954-962.
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