Adiponectin receptors gene expression and insulin sensitivity in non-diabetic Mexican Americans with or without a family history of Type 2 diabetes

A. E. Civitarese, C. P. Jenkinson, D. Richardson, M. Bajaj, K. Cusi, S. Kashyap, R. Berria, R. Belfort, R. A. DeFronzo, L. J. Mandarino, E. Ravussin

Research output: Contribution to journalArticle

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Abstract

Aims/hypothesis. The recent discovery of two adiponectin receptors (AdipoR1 and AdipoR2) will improve our understanding of the molecular mechanisms underlying the insulin-sensitising effect of adiponectin. The aim of this study was to determine for the first time whether skeletal muscle AdipoR1 and/or AdipoR2 gene expression levels are associated with insulin resistance. Methods. Using RT-PCR and northern analysis we measured AdipoR1 and AdipoR2 gene expression in skeletal muscle from healthy Mexican Americans with normal glucose tolerance who had (n=8) or did not have (n=10) a family history of Type 2 diabetes. Results. Gene expression profiling indicated that the AdipoR1 and AdipoR2 isoforms are highly expressed in human skeletal muscle, unlike in mice where AdipoR2 expression was highest in the liver, and AdipoR1 was highest in skeletal muscle. In the study subjects, the expression levels of AdipoR1 (p=0.004) and AdipoR2 (p=0.04), as well as plasma adiponectin concentration (p=0.03) were lower in people with a family history of Type 2 diabetes than in those with no family history of the disease. Importantly, the expression levels of both receptors correlated positively with insulin sensitivity (r=0.64, p=0.004 and r=0.47, p=0.048 respectively). Conclusions/interpretation. Collectively, these data indicate that both isoforms of the adiponectin receptor play a role in the insulin-sensitising effect of adiponectin.

Original languageEnglish (US)
Pages (from-to)816-820
Number of pages5
JournalDiabetologia
Volume47
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Adiponectin Receptors
Type 2 Diabetes Mellitus
Insulin Resistance
Adiponectin
Skeletal Muscle
Gene Expression
Protein Isoforms
Insulin
Gene Expression Profiling
Glucose
Polymerase Chain Reaction
Liver

Keywords

  • Adiponectin
  • AdipoR1
  • AdipoR2
  • Insulin resistance
  • Mexican American
  • Type 2 diabetes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Adiponectin receptors gene expression and insulin sensitivity in non-diabetic Mexican Americans with or without a family history of Type 2 diabetes. / Civitarese, A. E.; Jenkinson, C. P.; Richardson, D.; Bajaj, M.; Cusi, K.; Kashyap, S.; Berria, R.; Belfort, R.; DeFronzo, R. A.; Mandarino, L. J.; Ravussin, E.

In: Diabetologia, Vol. 47, No. 5, 05.2004, p. 816-820.

Research output: Contribution to journalArticle

Civitarese, AE, Jenkinson, CP, Richardson, D, Bajaj, M, Cusi, K, Kashyap, S, Berria, R, Belfort, R, DeFronzo, RA, Mandarino, LJ & Ravussin, E 2004, 'Adiponectin receptors gene expression and insulin sensitivity in non-diabetic Mexican Americans with or without a family history of Type 2 diabetes', Diabetologia, vol. 47, no. 5, pp. 816-820. https://doi.org/10.1007/s00125-004-1359-x
Civitarese, A. E. ; Jenkinson, C. P. ; Richardson, D. ; Bajaj, M. ; Cusi, K. ; Kashyap, S. ; Berria, R. ; Belfort, R. ; DeFronzo, R. A. ; Mandarino, L. J. ; Ravussin, E. / Adiponectin receptors gene expression and insulin sensitivity in non-diabetic Mexican Americans with or without a family history of Type 2 diabetes. In: Diabetologia. 2004 ; Vol. 47, No. 5. pp. 816-820.
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AU - Bajaj, M.

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AU - Kashyap, S.

AU - Berria, R.

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AU - Mandarino, L. J.

AU - Ravussin, E.

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