Adenine synthesis in a model prebiotic reaction: Connecting origin of life chemistry with biology

Lakshmi N. Anumukonda, Avery Young, David G. Lynn, Ragan Buckley, Amena Warrayat, Christina L. Graves, Heather Bean, Nicholas V. Hud

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Many high school laboratory experiments demonstrate concepts related to biological evolution, but few exist that allow students to investigate life's chemical origins. This series of laboratory experiments has been developed to allow students to explore and appreciate the deep connection that exists between prebiotic chemistry, chemical evolution, and contemporary biological systems. In the first experiment of the series, students synthesize adenine, one of the purine nucleobases of DNA and RNA, from plausibly prebiotic precursor molecules. Students compare their product to authentic standards using thin-layer chromatography. The second and third experiments of the series allow students to extract DNA from a familiar organism, the strawberry, and hydrolyze it, releasing adenine, which they can then compare to the previously chemically-synthesized adenine. A fourth, optional experiment is included where the technique of thin-layer chromatography is introduced and chromatographic skills are developed for use in the other three experiments that comprise this series. Concepts relating to organic and analytical chemistry, as well as biochemistry and DNA structure, are incorporated throughout, allowing this series of laboratory experiments to be easily inserted into existing laboratory courses and to reinforce concepts already included in any high school chemistry or biology curriculum.

Original languageEnglish (US)
Pages (from-to)1698-1701
Number of pages4
JournalJournal of Chemical Education
Volume88
Issue number12
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

Fingerprint

Prebiotics
Adenine
biology
chemistry
laboratory experiment
Students
experiment
student
Experiments
Thin layer chromatography
DNA
biochemistry
school
Biochemistry
Biological systems
Curricula
curriculum
RNA
Molecules
Chemical analysis

Keywords

  • Bioanalytical Chemistry
  • Biochemistry
  • Bioorganic Chemistry
  • First-Year Undergraduate/General
  • Hands-On Learning/Manipulatives
  • High School/Introductory ChemistryAnalytical Chemistry
  • Nucleic Acids/DNA/RNA
  • Organic Chemistry
  • Thin Layer Chromatography

ASJC Scopus subject areas

  • Chemistry(all)
  • Education

Cite this

Anumukonda, L. N., Young, A., Lynn, D. G., Buckley, R., Warrayat, A., Graves, C. L., ... Hud, N. V. (2011). Adenine synthesis in a model prebiotic reaction: Connecting origin of life chemistry with biology. Journal of Chemical Education, 88(12), 1698-1701. https://doi.org/10.1021/ed1012074

Adenine synthesis in a model prebiotic reaction : Connecting origin of life chemistry with biology. / Anumukonda, Lakshmi N.; Young, Avery; Lynn, David G.; Buckley, Ragan; Warrayat, Amena; Graves, Christina L.; Bean, Heather; Hud, Nicholas V.

In: Journal of Chemical Education, Vol. 88, No. 12, 01.12.2011, p. 1698-1701.

Research output: Contribution to journalArticle

Anumukonda, LN, Young, A, Lynn, DG, Buckley, R, Warrayat, A, Graves, CL, Bean, H & Hud, NV 2011, 'Adenine synthesis in a model prebiotic reaction: Connecting origin of life chemistry with biology', Journal of Chemical Education, vol. 88, no. 12, pp. 1698-1701. https://doi.org/10.1021/ed1012074
Anumukonda LN, Young A, Lynn DG, Buckley R, Warrayat A, Graves CL et al. Adenine synthesis in a model prebiotic reaction: Connecting origin of life chemistry with biology. Journal of Chemical Education. 2011 Dec 1;88(12):1698-1701. https://doi.org/10.1021/ed1012074
Anumukonda, Lakshmi N. ; Young, Avery ; Lynn, David G. ; Buckley, Ragan ; Warrayat, Amena ; Graves, Christina L. ; Bean, Heather ; Hud, Nicholas V. / Adenine synthesis in a model prebiotic reaction : Connecting origin of life chemistry with biology. In: Journal of Chemical Education. 2011 ; Vol. 88, No. 12. pp. 1698-1701.
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