TY - JOUR
T1 - Adaptation of Escherichia coli to long-term serial passage in complex medium
T2 - Evidence of parallel evolution
AU - Kram, Karin E.
AU - Geiger, Christopher
AU - Ismail, Wazim Mohammed
AU - Lee, Heewook
AU - Tang, Haixu
AU - Foster, Patricia L.
AU - Finkel, Steven E.
N1 - Funding Information:
This work was supported by U.S. Army Research Office grants (grant W911NF1010444 to P.L.F., H.T., and S.E.F. and W911NF1210321 to S.E.F.). K.E.K. was partially supported by the CSUDH Research, Scholarship, and Creative Activity award. H.L. was partially supported by grant R01HG007104 from the U.S. National Institutes of Health and grant GBMF4554 from the Gordon and Betty Moore Foundation to Carl Kingsford. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Publisher Copyright:
Copyright © 2017 Kram et al.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Experimental evolution of bacterial populations in the laboratory has led to identification of several themes, including parallel evolution of populations adapting to carbon starvation, heat stress, and pH stress. However, most of these experiments study growth in defined and/or constant environments. We hypothesized that while there would likely continue to be parallelism in more complex and changing environments, there would also be more variation in what types of mutations would benefit the cells. In order to test our hypothesis, we serially passaged Escherichia coli in a complex medium (Luria-Bertani broth) throughout the five phases of bacterial growth. This passaging scheme allowed cells to experience a wide variety of stresses, including nutrient limitation, oxidative stress, and pH variation, and therefore allowed them to adapt to several conditions. After every ∼30 generations of growth, for a total of ∼300 generations, we compared both the growth phenotypes and genotypes of aged populations to the parent population. After as few as 30 generations, populations exhibit changes in growth phenotype and accumulate potentially adaptive mutations. There were many genes with mutant alleles in different populations, indicating potential parallel evolution. We examined 8 of these alleles by constructing the point mutations in the parental genetic background and competed those cells with the parent population; five of these alleles were found to be adaptive. The variety and swiftness of adaptive mutations arising in the populations indicate that the cells are adapting to a complex set of stresses, while the parallel nature of several of the mutations indicates that this behavior may be generalized to bacterial evolution.
AB - Experimental evolution of bacterial populations in the laboratory has led to identification of several themes, including parallel evolution of populations adapting to carbon starvation, heat stress, and pH stress. However, most of these experiments study growth in defined and/or constant environments. We hypothesized that while there would likely continue to be parallelism in more complex and changing environments, there would also be more variation in what types of mutations would benefit the cells. In order to test our hypothesis, we serially passaged Escherichia coli in a complex medium (Luria-Bertani broth) throughout the five phases of bacterial growth. This passaging scheme allowed cells to experience a wide variety of stresses, including nutrient limitation, oxidative stress, and pH variation, and therefore allowed them to adapt to several conditions. After every ∼30 generations of growth, for a total of ∼300 generations, we compared both the growth phenotypes and genotypes of aged populations to the parent population. After as few as 30 generations, populations exhibit changes in growth phenotype and accumulate potentially adaptive mutations. There were many genes with mutant alleles in different populations, indicating potential parallel evolution. We examined 8 of these alleles by constructing the point mutations in the parental genetic background and competed those cells with the parent population; five of these alleles were found to be adaptive. The variety and swiftness of adaptive mutations arising in the populations indicate that the cells are adapting to a complex set of stresses, while the parallel nature of several of the mutations indicates that this behavior may be generalized to bacterial evolution.
KW - Adaptive evolution
KW - Laboratory evolution
KW - Long-term stationary phase
KW - Survival
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U2 - 10.1128/mSystems.00192-16
DO - 10.1128/mSystems.00192-16
M3 - Article
AN - SCOPUS:85020555093
SN - 2379-5077
VL - 2
JO - mSystems
JF - mSystems
IS - 2
M1 - e00192
ER -