Abstract
An immunosensing method is described for the measurement of γ-amino-4-butyric acid (GABA), one of two inhibitory neurotransmitters in the central nervous system. Monoclonal antibody for GABA was immobilized on the gold electrodes of quartz crystal. Binding of GABA produced a decrease in the resonant frequency of quartz crystal. In in-vitro experiments, the sensor demonstrated a sensitivity of 600Hz decrease in resonant frequency in response to 49 mM change in GABA concentration. The response time was less than 10 seconds. The binding sites of immobilized antibody were successfully regenerated by repeated washing with ImM HCL solution. A significant increase in the resistance of the electrical model of the quartz crystal indicated an increase in the viscosity due to the binding of GABA to the anti-body. An acoustic immunosensor sensitive and specific to GABA was demonstrated. Real-time sensing of GABA will enable greater insights into several neuropathologies like epilepsy, Parkinson's disease etc. where the role of GABA is critical.
| Original language | English (US) |
|---|---|
| Title of host publication | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Editors | R.S. Leder |
| Pages | 2998-3000 |
| Number of pages | 3 |
| Volume | 4 |
| State | Published - 2003 |
| Event | A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Cancun, Mexico Duration: Sep 17 2003 → Sep 21 2003 |
Other
| Other | A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society |
|---|---|
| Country/Territory | Mexico |
| City | Cancun |
| Period | 9/17/03 → 9/21/03 |
Keywords
- Biosensor
- GABA
- Monoclonal anti-GABA antibody
- QCM
ASJC Scopus subject areas
- Bioengineering