Achieving superior carbon transfer efficiency and pH control using membrane carbonation with a wide range of CO₂ contents for the coccolithophore Emiliania huxleyi

The economic viability of microalgal-derived products relies on rapid CO₂ transfer in a cost-effective manner. Many industrial gas streams contain concentrated CO₂ that, if converted to useful products, would lower greenhouse gas emissions and valorize the wasted CO₂. Membrane carbonation (MC) uses non-porous hollow-fiber gas-transfer membranes to deliver CO₂ without bubble formation, which makes it possible to achieve a high carbon-transfer efficiency (CTE). However, inert gasses in the industrial streams (e.g., N₂, O₂, and H₂O) can significantly lower the CO₂-delivery rate. The means to overcome the buildup of inert gases in the membrane lumen is to manage the distal end of the membranes to sweep out inert gases while not wasting significant CO₂. A MC-venting strategy was evaluated for CO₂ inputs from 5% to 100%. Abiotic tests using a restricted exit flow could achieve >95% CTE_abiotic for industrial CO₂ streams. When integrated with semi-continuous cultivation of a marine coccolithophore, Emiliania huxleyi, CO₂ delivery and venting were on-demand based on a pH set points and pH-actuated feed and venting valves. MC using the venting strategy achieved 100% CTE_biotic when delivering 100% and 50% CO₂, which was better than 50% CTE_biotic obtained from pH-controlled sparging of 100% CO₂-sparging. E. huxleyi consistently fixed ~80% of the delivered CO₂ into biomass, and the remaining ~20% to calcite coccoliths. The compact size of MC modules, stable pH control, and no shear forces from bubble agitation during the CO₂ delivery made MC an ideal match for cultivation of coccolithophores, which are sensitive to shear forces and pH fluctuations.