TY - JOUR
T1 - Achieving High Spatial Resolution Surface Plasmon Resonance Microscopy with Image Reconstruction
AU - Yu, Hui
AU - Shan, Xiaonan
AU - Wang, Shaopeng
AU - Tao, Nongjian
N1 - Funding Information:
We thank National Natural Science Foundation of China (Grant No. 21327008) and Gordon and Betty Moore Foundation for financial support.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/3/7
Y1 - 2017/3/7
N2 - Surface plasmon resonance microscopy (SPRM) is a powerful platform for biomedical imaging and molecular binding kinetics analysis. However, the spatial resolution of SPRM along the plasmon propagation direction (longitudinal) is determined by the decaying length of the plasmonic wave, which can be as large as tens of microns. Different methods have been proposed to improve the spatial resolution, but each at the expense of decreased sensitivity or temporal resolution. Here we present a method to achieve high spatial resolution SPRM based on deconvolution of complex field. The method does not require additional optical setup and improves the spatial resolution in the longitudinal direction. We applied the method to image nanoparticles and achieved close-to-diffraction limit resolution in both longitudinal and transverse directions.
AB - Surface plasmon resonance microscopy (SPRM) is a powerful platform for biomedical imaging and molecular binding kinetics analysis. However, the spatial resolution of SPRM along the plasmon propagation direction (longitudinal) is determined by the decaying length of the plasmonic wave, which can be as large as tens of microns. Different methods have been proposed to improve the spatial resolution, but each at the expense of decreased sensitivity or temporal resolution. Here we present a method to achieve high spatial resolution SPRM based on deconvolution of complex field. The method does not require additional optical setup and improves the spatial resolution in the longitudinal direction. We applied the method to image nanoparticles and achieved close-to-diffraction limit resolution in both longitudinal and transverse directions.
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U2 - 10.1021/acs.analchem.6b05049
DO - 10.1021/acs.analchem.6b05049
M3 - Article
C2 - 28194944
AN - SCOPUS:85026204030
SN - 0003-2700
VL - 89
SP - 2704
EP - 2707
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 5
ER -