Acetylcholinesterase complexation with acetylthiocholine or organophosphate at the air/aqueous interface: AFM and UV-vis studies

Leila Dziri, Salah Boussaad, Nongjian Tao, Roger M. Leblanc

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The hydrolysis reaction of acetylthiocholine catalysed by the enzyme, acetylcholinesterase (AChE), was studied at the air/aqueous interface by spreading the enzyme as a monolayer and dissolving the substrate in the snbphase The reaction progress was monitored by time-dependent UV-vis. and the topography of the Langmuir- Blodgett fillms was determined by tapping mode atomic force microscopy (TMAFM). For a better understanding of the complex formation mechanism between AChE and its substrate, acetylthiocholine, the AChE monolayer was prepared and examined with TXAFM in two steps. The monolayer was first compressed on the substrate-free buffered subphase. Once a surface pressure of 25 mN/m was reached, the acetyithiocholine was injected into the subphase. The TMAFM images of a transferred monolayer, 6 min after the injection, show the presence of an acetylcholinesterase-acetylthiocholine complex and a homogeneous monolayer composition. However, the images of a second transferred monolayer at the same surface pressure, but 15 min after the injection, indicate the formation of a mixed monolayer due to the presence of both the enzyme-substrate complex and the free enzyme. Compression of the AChE monolayer on a substrate subphase indicates that the hydrolysis reaction took place at the interface and ended before a surface pressurs of mN/m was reached. Therefore, the topography of a monolayer prepared on a subphase containing the substrate resulted in a heterogenesous surface structure doe to the presence of free enzymes and reaction products. UV-vis data confirmed the observations deduced from the TMAFM images. Furthermore, the effect of the organophosphate, paraoxon, on the enzyme was studied at the air/aqueous and the air/solid interfaces. The structural conformation of the enzyme is altered significantly by the presence of the inhibitor. Large domains were observed rather than an organized acetylcholinesterase monolayer, and the spectroscopic properties indicate that the interaction between the acetylcholinesterase and the paraoxon took place at the air/aqueous interface.

Original languageEnglish (US)
Pages (from-to)4853-4859
Number of pages7
JournalLangmuir
Volume14
Issue number17
StatePublished - Aug 18 1998
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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