Accelerating the rate of cometabolic degradations requiring an intracellular electron source-model and biofilm application

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8 Citations (Scopus)

Abstract

This paper applies our recently acquired knowledge of the large and systematic changes in internal reducing power to controlled changes in the cell's primary electron-donor and -acceptor substrates. The systematic cellular responses of the NADH/NAD ratio is incorporated into kinetic equations for reductive dehalogenation and oxygenation reactions. Results show that the external donor and acceptor concentrations strongly affect the percentage removal of hazardous compounds. The simplest strategy for maximizing the efficiency of reductive dehalogenation is to maintain a saturating concentration of the primary electron donor, or, as a next best alternative, to minimize the concentration of electron accepters. Fur mono- or dioxygenation reactions, consistently high percentage removals can be achieved when the concentrations of both primary substrates are high. Removals of carbon tetrachloride in denitrifying biofilm experiments are in perfect accord with the model predictions for reductive dehalogenation. They show a dramatic increase in percentage removal when the primary electron acceptor (NO3 -) is removed from the reactor and a substantial decrease when the electron donor (acetate) is removed. Thus, the biofilm experiments verify that biodegradation reactions can be accelerated dramatically by manipulation of primary donor and acceptor concentrations and in a manner consistent with modeling predictions based on the internal reducing power.

Original languageEnglish (US)
Pages (from-to)29-39
Number of pages11
JournalWater Science and Technology
Volume31
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Electron sources
Biofilms
biofilm
Dehalogenation
Degradation
electron
degradation
Electrons
substrate
Carbon tetrachloride
Oxygenation
oxygenation
Substrates
prediction
Biodegradation
biodegradation
acetate
experiment
Experiments
rate

Keywords

  • Biofilm
  • Electron acceptor
  • Electron donor
  • Kinetics
  • Oxygenation
  • Reducing power
  • Reductive dehalogenation
  • Secondary utilization
  • Solvents

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

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title = "Accelerating the rate of cometabolic degradations requiring an intracellular electron source-model and biofilm application",
abstract = "This paper applies our recently acquired knowledge of the large and systematic changes in internal reducing power to controlled changes in the cell's primary electron-donor and -acceptor substrates. The systematic cellular responses of the NADH/NAD ratio is incorporated into kinetic equations for reductive dehalogenation and oxygenation reactions. Results show that the external donor and acceptor concentrations strongly affect the percentage removal of hazardous compounds. The simplest strategy for maximizing the efficiency of reductive dehalogenation is to maintain a saturating concentration of the primary electron donor, or, as a next best alternative, to minimize the concentration of electron accepters. Fur mono- or dioxygenation reactions, consistently high percentage removals can be achieved when the concentrations of both primary substrates are high. Removals of carbon tetrachloride in denitrifying biofilm experiments are in perfect accord with the model predictions for reductive dehalogenation. They show a dramatic increase in percentage removal when the primary electron acceptor (NO3 -) is removed from the reactor and a substantial decrease when the electron donor (acetate) is removed. Thus, the biofilm experiments verify that biodegradation reactions can be accelerated dramatically by manipulation of primary donor and acceptor concentrations and in a manner consistent with modeling predictions based on the internal reducing power.",
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AB - This paper applies our recently acquired knowledge of the large and systematic changes in internal reducing power to controlled changes in the cell's primary electron-donor and -acceptor substrates. The systematic cellular responses of the NADH/NAD ratio is incorporated into kinetic equations for reductive dehalogenation and oxygenation reactions. Results show that the external donor and acceptor concentrations strongly affect the percentage removal of hazardous compounds. The simplest strategy for maximizing the efficiency of reductive dehalogenation is to maintain a saturating concentration of the primary electron donor, or, as a next best alternative, to minimize the concentration of electron accepters. Fur mono- or dioxygenation reactions, consistently high percentage removals can be achieved when the concentrations of both primary substrates are high. Removals of carbon tetrachloride in denitrifying biofilm experiments are in perfect accord with the model predictions for reductive dehalogenation. They show a dramatic increase in percentage removal when the primary electron acceptor (NO3 -) is removed from the reactor and a substantial decrease when the electron donor (acetate) is removed. Thus, the biofilm experiments verify that biodegradation reactions can be accelerated dramatically by manipulation of primary donor and acceptor concentrations and in a manner consistent with modeling predictions based on the internal reducing power.

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KW - Secondary utilization

KW - Solvents

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