ACAPELLA, a capillary-based submicroliter automated sample preparation system for genome analysis

Deirdre Meldrum, Harold T. Evensen, William H. Pence, Stephen E. Moody, David L. Cunningham, Peter Wiktor

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The Genomation Laboratory in the Electrical Engineering Department at the University of Washington has been developing an automated, high-throughput submicroliter-scale reaction preparation system for use in molecular biology, especially as part of the Human Genome Project and other high-throughput DNA sequencing endeavors. Small glass capillaries enable the preparation, handling and monitoring of one-microliter reaction volumes. The Genomation Laboratory, with corporate partners Orca Photonic Systems, Inc. and Engineering Arts, has developed modules for aspiration, dispensing, mixing, transport and rapid thermal treatment of biological samples contained in glass capillaries. The `ACAPELLA 1K' is the first integration of these modules, designed to process 1,000 samples in an 8-hour day. It has served as a test bed for the technologies as well as for performing biological experiments in conjunction with the University of Washington Genome Center. The Genomation Laboratory is presently developing the next-stage `ACAPELLA 5K' system based on the results of the ACAPELLA 1K system.

Original languageEnglish (US)
Title of host publicationIEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages39-48
Number of pages10
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE/ASME International Conference on Advanced Intelligent Mechatronics (AIM '99) - Atlanta, GA, USA
Duration: Sep 19 1999Sep 23 1999

Other

OtherProceedings of the 1999 IEEE/ASME International Conference on Advanced Intelligent Mechatronics (AIM '99)
CityAtlanta, GA, USA
Period9/19/999/23/99

    Fingerprint

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Meldrum, D., Evensen, H. T., Pence, W. H., Moody, S. E., Cunningham, D. L., & Wiktor, P. (1999). ACAPELLA, a capillary-based submicroliter automated sample preparation system for genome analysis. In IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM (pp. 39-48). IEEE.