ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System

Deirdre Meldrum; Charles H. Fisher; Matthew P. Moore; Mohan Saini; Mark R. Holl; William H. Pence; Stephen E. Moody; David L. Cunningham; Peter Wiktor

ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System

Deirdre Meldrum, Charles H. Fisher, Matthew P. Moore, Mohan Saini, Mark R. Holl, William H. Pence, Stephen E. Moody, David L. Cunningham, Peter Wiktor

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

8 Scopus citations

Abstract

A capillary-based fluid handling system has been developed to process 5000 samples in 8 hours. The system takes deoxyribonucleic acid (DNA) or other chemical samples and automatically processes them as specified in a user-defined protocol with aspiration, dispensing, mixing, thermal cycling, and imaging steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the samples prepared. This laboratory automation system dispenses 40-100 pL droplet volumes using piezoelectric reagent dispensers and prepares 1 or 2-μL final reaction volumes, a 3 to 5-fold reduction in reagent usage over current (2003) state-of-the-art manual and automated instrumentation. Extensive testing of the system has been performed with the University of Washington Genome Center. Applications for ACAPELLA-5K include DNA sequencing, diagnostics, minimal residual disease quantification, drug discovery, environmental testing, forensics, protein crystallography, Polymerase Chain Reaction (PCR) and so on.

Original language	English (US)
Title of host publication	IEEE International Conference on Intelligent Robots and Systems
Pages	2321-2328
Number of pages	8
Volume	3
State	Published - 2003
Externally published	Yes
Event	2003 IEEE/RSJ International Conference on Intelligent Robots and Systems - Las Vegas, NV, United States Duration: Oct 27 2003 → Oct 31 2003

Other

Other	2003 IEEE/RSJ International Conference on Intelligent Robots and Systems
Country/Territory	United States
City	Las Vegas, NV
Period	10/27/03 → 10/31/03

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Meldrum, D, Fisher, CH, Moore, MP, Saini, M, Holl, MR, Pence, WH, Moody, SE, Cunningham, DL & Wiktor, P 2003, ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System. in IEEE International Conference on Intelligent Robots and Systems. vol. 3, pp. 2321-2328, 2003 IEEE/RSJ International Conference on Intelligent Robots and Systems, Las Vegas, NV, United States, 10/27/03.

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abstract = "A capillary-based fluid handling system has been developed to process 5000 samples in 8 hours. The system takes deoxyribonucleic acid (DNA) or other chemical samples and automatically processes them as specified in a user-defined protocol with aspiration, dispensing, mixing, thermal cycling, and imaging steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the samples prepared. This laboratory automation system dispenses 40-100 pL droplet volumes using piezoelectric reagent dispensers and prepares 1 or 2-μL final reaction volumes, a 3 to 5-fold reduction in reagent usage over current (2003) state-of-the-art manual and automated instrumentation. Extensive testing of the system has been performed with the University of Washington Genome Center. Applications for ACAPELLA-5K include DNA sequencing, diagnostics, minimal residual disease quantification, drug discovery, environmental testing, forensics, protein crystallography, Polymerase Chain Reaction (PCR) and so on.",

author = "Deirdre Meldrum and Fisher, {Charles H.} and Moore, {Matthew P.} and Mohan Saini and Holl, {Mark R.} and Pence, {William H.} and Moody, {Stephen E.} and Cunningham, {David L.} and Peter Wiktor",

year = "2003",

language = "English (US)",

volume = "3",

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AU - Meldrum, Deirdre

AU - Fisher, Charles H.

AU - Moore, Matthew P.

AU - Saini, Mohan

AU - Holl, Mark R.

AU - Pence, William H.

AU - Moody, Stephen E.

AU - Cunningham, David L.

AU - Wiktor, Peter

PY - 2003

Y1 - 2003

N2 - A capillary-based fluid handling system has been developed to process 5000 samples in 8 hours. The system takes deoxyribonucleic acid (DNA) or other chemical samples and automatically processes them as specified in a user-defined protocol with aspiration, dispensing, mixing, thermal cycling, and imaging steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the samples prepared. This laboratory automation system dispenses 40-100 pL droplet volumes using piezoelectric reagent dispensers and prepares 1 or 2-μL final reaction volumes, a 3 to 5-fold reduction in reagent usage over current (2003) state-of-the-art manual and automated instrumentation. Extensive testing of the system has been performed with the University of Washington Genome Center. Applications for ACAPELLA-5K include DNA sequencing, diagnostics, minimal residual disease quantification, drug discovery, environmental testing, forensics, protein crystallography, Polymerase Chain Reaction (PCR) and so on.

AB - A capillary-based fluid handling system has been developed to process 5000 samples in 8 hours. The system takes deoxyribonucleic acid (DNA) or other chemical samples and automatically processes them as specified in a user-defined protocol with aspiration, dispensing, mixing, thermal cycling, and imaging steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the samples prepared. This laboratory automation system dispenses 40-100 pL droplet volumes using piezoelectric reagent dispensers and prepares 1 or 2-μL final reaction volumes, a 3 to 5-fold reduction in reagent usage over current (2003) state-of-the-art manual and automated instrumentation. Extensive testing of the system has been performed with the University of Washington Genome Center. Applications for ACAPELLA-5K include DNA sequencing, diagnostics, minimal residual disease quantification, drug discovery, environmental testing, forensics, protein crystallography, Polymerase Chain Reaction (PCR) and so on.

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M3 - Conference contribution

AN - SCOPUS:0348252374

VL - 3

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EP - 2328

BT - IEEE International Conference on Intelligent Robots and Systems

T2 - 2003 IEEE/RSJ International Conference on Intelligent Robots and Systems

Y2 - 27 October 2003 through 31 October 2003

ER -

ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System

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ASJC Scopus subject areas

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