ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System

Deirdre Meldrum, Charles H. Fisher, Matthew P. Moore, Mohan Saini, Mark R. Holl, William H. Pence, Stephen E. Moody, David L. Cunningham, Peter Wiktor

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

A capillary-based fluid handling system has been developed to process 5000 samples in 8 hours. The system takes deoxyribonucleic acid (DNA) or other chemical samples and automatically processes them as specified in a user-defined protocol with aspiration, dispensing, mixing, thermal cycling, and imaging steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the samples prepared. This laboratory automation system dispenses 40-100 pL droplet volumes using piezoelectric reagent dispensers and prepares 1 or 2-μL final reaction volumes, a 3 to 5-fold reduction in reagent usage over current (2003) state-of-the-art manual and automated instrumentation. Extensive testing of the system has been performed with the University of Washington Genome Center. Applications for ACAPELLA-5K include DNA sequencing, diagnostics, minimal residual disease quantification, drug discovery, environmental testing, forensics, protein crystallography, Polymerase Chain Reaction (PCR) and so on.

Original languageEnglish (US)
Title of host publicationIEEE International Conference on Intelligent Robots and Systems
Pages2321-2328
Number of pages8
Volume3
StatePublished - 2003
Externally publishedYes
Event2003 IEEE/RSJ International Conference on Intelligent Robots and Systems - Las Vegas, NV, United States
Duration: Oct 27 2003Oct 31 2003

Other

Other2003 IEEE/RSJ International Conference on Intelligent Robots and Systems
CountryUnited States
CityLas Vegas, NV
Period10/27/0310/31/03

Fingerprint

DNA
Genes
Throughput
Environmental testing
Dispensers
Crystallography
Polymerase chain reaction
Infrared imaging
Thermal cycling
Chemical analysis
Automation
Pipelines
Proteins
Fluids
Testing
Drug Discovery

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Meldrum, D., Fisher, C. H., Moore, M. P., Saini, M., Holl, M. R., Pence, W. H., ... Wiktor, P. (2003). ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System. In IEEE International Conference on Intelligent Robots and Systems (Vol. 3, pp. 2321-2328)

ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System. / Meldrum, Deirdre; Fisher, Charles H.; Moore, Matthew P.; Saini, Mohan; Holl, Mark R.; Pence, William H.; Moody, Stephen E.; Cunningham, David L.; Wiktor, Peter.

IEEE International Conference on Intelligent Robots and Systems. Vol. 3 2003. p. 2321-2328.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Meldrum, D, Fisher, CH, Moore, MP, Saini, M, Holl, MR, Pence, WH, Moody, SE, Cunningham, DL & Wiktor, P 2003, ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System. in IEEE International Conference on Intelligent Robots and Systems. vol. 3, pp. 2321-2328, 2003 IEEE/RSJ International Conference on Intelligent Robots and Systems, Las Vegas, NV, United States, 10/27/03.
Meldrum D, Fisher CH, Moore MP, Saini M, Holl MR, Pence WH et al. ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System. In IEEE International Conference on Intelligent Robots and Systems. Vol. 3. 2003. p. 2321-2328
Meldrum, Deirdre ; Fisher, Charles H. ; Moore, Matthew P. ; Saini, Mohan ; Holl, Mark R. ; Pence, William H. ; Moody, Stephen E. ; Cunningham, David L. ; Wiktor, Peter. / ACAPELLA-5K, A High-Throughput Automated Genome and Chemical Analysis System. IEEE International Conference on Intelligent Robots and Systems. Vol. 3 2003. pp. 2321-2328
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