Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes

Justin York, David Spetzler, Tassilo Hornung, Robert Ishmukhametov, James Martin, Wayne Frasch

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The abundance of E. coli F1-ATPase molecules observed to rotate using gold nanorods attached to the γ-subunit was quantitated. Individual F1 molecules were determined to be rotating based upon time dependent fluctuations of red and green light scattered from the nanorods when viewed through a polarizing filter. The average number of F1 molecules observed to rotate in the presence of GTP, ATP, and without nucleotide was ∼50, ∼25, and ∼4% respectively. In some experiments, the fraction of molecules observed to rotate in the presence of GTP was as high as 65%. These data indicate that rotational measurements made using gold nanorods provide information of the F1-ATPase mechanism that is representative of the characteristics of the enzyme population as a whole.

Original languageEnglish (US)
Pages (from-to)435-439
Number of pages5
JournalJournal of Bioenergetics and Biomembranes
Volume39
Issue number5-6
DOIs
StatePublished - Dec 1 2007

Keywords

  • F-ATPase
  • Gold nanorods
  • Molecular motors
  • Plasmon resonance
  • Single molecule microscopy

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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