Abrupt buckling transition observed during the plectoneme formation of individual DNA molecules

Scott Forth, Christopher Deufel, Maxim Y. Sheinin, BRYAN DANIELS, James P. Sethna, Michelle D. Wang

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

The response of single DNA molecules to externally applied forces and torques was directly measured using an angular optical trap. Upon overwinding, DNA buckled abruptly as revealed by a sharp extension drop followed by a torque plateau. When the DNA was held at the buckling transition, its extension hopped rapidly between two distinct states. Furthermore, the initial plectonemic loop absorbed approximately twice as much extension as was absorbed into the plectoneme upon each additional turn. The observed extension change after buckling and the postbuckling torque support a recent DNA elasticity model.

Original languageEnglish (US)
Article number148301
JournalPhysical Review Letters
Volume100
Issue number14
DOIs
StatePublished - Apr 8 2008
Externally publishedYes

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buckling
deoxyribonucleic acid
torque
molecules
plateaus
elastic properties
traps

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Abrupt buckling transition observed during the plectoneme formation of individual DNA molecules. / Forth, Scott; Deufel, Christopher; Sheinin, Maxim Y.; DANIELS, BRYAN; Sethna, James P.; Wang, Michelle D.

In: Physical Review Letters, Vol. 100, No. 14, 148301, 08.04.2008.

Research output: Contribution to journalArticle

Forth, Scott ; Deufel, Christopher ; Sheinin, Maxim Y. ; DANIELS, BRYAN ; Sethna, James P. ; Wang, Michelle D. / Abrupt buckling transition observed during the plectoneme formation of individual DNA molecules. In: Physical Review Letters. 2008 ; Vol. 100, No. 14.
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