Abstract
A much larger sample (N = 2369) was used to evaluate a previously reported distribution of the A, AB and B blood group phenotypes in rhesus and cynomolgus macaques from six different regional populations. These samples, acquired from 15 different breeding and research facilities in the United States, were analyzed using a real-time quantitative polymerase chain reaction (qPCR) assay that targets single nucleotide polymorphisms (SNPs) responsible for the macaque A, B and AB phenotypes. The frequency distributions of blood group phenotypes of the two species differ significantly from each other and significant regional differentiation within the geographic ranges of each species was also observed. The B blood group phenotype was prevalent in rhesus macaques, especially those from India, while the frequencies of the A, B and AB phenotypes varied significantly among cynomolgus macaques from different geographic regions. The Mauritian cynomolgus macaques, despite having originated in Indonesia, showed significant (P ≪.01) divergence from the Indonesian animals at the ABO blood group locus. Most Mauritian animals belonged to the B blood group while the Indonesian animals were mostly A. The close similarity in blood group frequency distributions between the Chinese rhesus and Indochinese cynomolgus macaques demonstrates that the introgression between these two species extends beyond the zone of intergradation in Indochina. This study underscores the importance of ABO blood group phenotyping of the domestic supply of macaques and their biospecimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 295-299 |
| Number of pages | 5 |
| Journal | HLA |
| Volume | 90 |
| Issue number | 5 |
| DOIs | |
| State | Published - Nov 1 2017 |
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Keywords
- ABO blood testing
- colony genetic management
- cynomolgus or long-tailed macaques
- molecular diagnostics
- rhesus macaques
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Genetics
Cite this
ABO blood group phenotype frequency estimation using molecular phenotyping in rhesus and cynomolgus macaques. / Kanthaswamy, Sreetharan; Ng, J.; Oldt, R. F.; Valdivia, L.; Houghton, P.; Smith, D. G.
In: HLA, Vol. 90, No. 5, 01.11.2017, p. 295-299.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ABO blood group phenotype frequency estimation using molecular phenotyping in rhesus and cynomolgus macaques
AU - Kanthaswamy, Sreetharan
AU - Ng, J.
AU - Oldt, R. F.
AU - Valdivia, L.
AU - Houghton, P.
AU - Smith, D. G.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - A much larger sample (N = 2369) was used to evaluate a previously reported distribution of the A, AB and B blood group phenotypes in rhesus and cynomolgus macaques from six different regional populations. These samples, acquired from 15 different breeding and research facilities in the United States, were analyzed using a real-time quantitative polymerase chain reaction (qPCR) assay that targets single nucleotide polymorphisms (SNPs) responsible for the macaque A, B and AB phenotypes. The frequency distributions of blood group phenotypes of the two species differ significantly from each other and significant regional differentiation within the geographic ranges of each species was also observed. The B blood group phenotype was prevalent in rhesus macaques, especially those from India, while the frequencies of the A, B and AB phenotypes varied significantly among cynomolgus macaques from different geographic regions. The Mauritian cynomolgus macaques, despite having originated in Indonesia, showed significant (P ≪.01) divergence from the Indonesian animals at the ABO blood group locus. Most Mauritian animals belonged to the B blood group while the Indonesian animals were mostly A. The close similarity in blood group frequency distributions between the Chinese rhesus and Indochinese cynomolgus macaques demonstrates that the introgression between these two species extends beyond the zone of intergradation in Indochina. This study underscores the importance of ABO blood group phenotyping of the domestic supply of macaques and their biospecimens.
AB - A much larger sample (N = 2369) was used to evaluate a previously reported distribution of the A, AB and B blood group phenotypes in rhesus and cynomolgus macaques from six different regional populations. These samples, acquired from 15 different breeding and research facilities in the United States, were analyzed using a real-time quantitative polymerase chain reaction (qPCR) assay that targets single nucleotide polymorphisms (SNPs) responsible for the macaque A, B and AB phenotypes. The frequency distributions of blood group phenotypes of the two species differ significantly from each other and significant regional differentiation within the geographic ranges of each species was also observed. The B blood group phenotype was prevalent in rhesus macaques, especially those from India, while the frequencies of the A, B and AB phenotypes varied significantly among cynomolgus macaques from different geographic regions. The Mauritian cynomolgus macaques, despite having originated in Indonesia, showed significant (P ≪.01) divergence from the Indonesian animals at the ABO blood group locus. Most Mauritian animals belonged to the B blood group while the Indonesian animals were mostly A. The close similarity in blood group frequency distributions between the Chinese rhesus and Indochinese cynomolgus macaques demonstrates that the introgression between these two species extends beyond the zone of intergradation in Indochina. This study underscores the importance of ABO blood group phenotyping of the domestic supply of macaques and their biospecimens.
KW - ABO blood testing
KW - colony genetic management
KW - cynomolgus or long-tailed macaques
KW - molecular diagnostics
KW - rhesus macaques
UR - http://www.scopus.com/inward/record.url?scp=85030702112&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85030702112&partnerID=8YFLogxK
U2 - 10.1111/tan.13118
DO - 10.1111/tan.13118
M3 - Article
C2 - 28800212
AN - SCOPUS:85030702112
VL - 90
SP - 295
EP - 299
JO - HLA
JF - HLA
SN - 2059-2302
IS - 5
ER -