Aberrant cannabinoid signaling impairs oviductal transport of embryos

Haibin Wang, Yong Guo, Dingzhi Wang, Philip J. Kingsley, Lawrence J. Marnett, Sanjoy K. Das, Raymond N. DuBois, Sudhansu K. Dey

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Ectopic pregnancy is a major reproductive health issue. Although other underlying causes remain largely unknown, one cause of ectopic pregnancy is embryo retention in the fallopian tube. Here we show that genetic or pharmacologic silencing of cannabinoid receptor CB1 causes retention of a large number of embryos in the mouse oviduct, eventually leading to pregnancy failure. This is reversed by isoproterenol, a β-adrenergic receptor agonist. Impaired oviductal embryo transport is also observed in wild-type mice treated with methanandamide. Collectively, the results suggest that aberrant cannabinoid signaling impedes coordinated oviductal smooth muscle contraction and relaxation crucial to normal oviductal embryo transport. Colocalization of CB1 and β2-adrenergic receptors in the oviduct muscularis implies that a basal endocannabinoid tone in collaboration with adrenergic receptors coordinates oviductal motility for normal journey of embryos into the uterus. Besides uncovering a new regulatory mechanism, this study could be clinically relevant to ectopic pregnancy.

Original languageEnglish (US)
Pages (from-to)1074-1080
Number of pages7
JournalNature Medicine
Volume10
Issue number10
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Cannabinoids
Adrenergic Receptors
Embryonic Structures
Ectopic Pregnancy
Cannabinoid Receptor CB1
Endocannabinoids
Adrenergic Agonists
Isoproterenol
Oviducts
Muscle
Health
Muscle Relaxation
Fallopian Tubes
Reproductive Health
Muscle Contraction
Uterus
Smooth Muscle
Pregnancy
methanandamide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Wang, H., Guo, Y., Wang, D., Kingsley, P. J., Marnett, L. J., Das, S. K., ... Dey, S. K. (2004). Aberrant cannabinoid signaling impairs oviductal transport of embryos. Nature Medicine, 10(10), 1074-1080. https://doi.org/10.1038/nm1104

Aberrant cannabinoid signaling impairs oviductal transport of embryos. / Wang, Haibin; Guo, Yong; Wang, Dingzhi; Kingsley, Philip J.; Marnett, Lawrence J.; Das, Sanjoy K.; DuBois, Raymond N.; Dey, Sudhansu K.

In: Nature Medicine, Vol. 10, No. 10, 10.2004, p. 1074-1080.

Research output: Contribution to journalArticle

Wang, H, Guo, Y, Wang, D, Kingsley, PJ, Marnett, LJ, Das, SK, DuBois, RN & Dey, SK 2004, 'Aberrant cannabinoid signaling impairs oviductal transport of embryos', Nature Medicine, vol. 10, no. 10, pp. 1074-1080. https://doi.org/10.1038/nm1104
Wang H, Guo Y, Wang D, Kingsley PJ, Marnett LJ, Das SK et al. Aberrant cannabinoid signaling impairs oviductal transport of embryos. Nature Medicine. 2004 Oct;10(10):1074-1080. https://doi.org/10.1038/nm1104
Wang, Haibin ; Guo, Yong ; Wang, Dingzhi ; Kingsley, Philip J. ; Marnett, Lawrence J. ; Das, Sanjoy K. ; DuBois, Raymond N. ; Dey, Sudhansu K. / Aberrant cannabinoid signaling impairs oviductal transport of embryos. In: Nature Medicine. 2004 ; Vol. 10, No. 10. pp. 1074-1080.
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