A549 lung epithelial cells grown as three-dimensional aggregates: Alternative tissue culture model for Pseudomonas aeruginosa pathogenesis

A. J. Carterson, K. Höner Zu Bentrup, C. M. Ott, M. S. Clarke, D. L. Pierson, C. R. Vanderburg, K. L. Buchanan, Cheryl Nickerson, M. J. Schurr

Research output: Contribution to journalArticle

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Abstract

A three-dimensional (3-D) lung aggregate model was developed from A549 human lung epithelial cells by using a rotating-wall vessel bioreactor to study the interactions between Pseudomonas aeruginosa and lung epithelial cells. The suitability of the 3-D aggregates as an infection model was examined by immunohistochemistry, adherence and invasion assays, scanning electron microscopy, and cytokine and mucoglycoprotein production. Immunohistochemical characterization of the 3-D A549 aggregates showed increased expression of epithelial cell-specific markers and decreased expression of cancer-specific markers compared to their monolayer counterparts. Immunohistochemistry of junctional markers on A549 3-D cells revealed that these cells formed tight junctions and polarity, in contrast to the cells grown as monolayers. Additionally, the 3-D aggregates stained positively for the production of mucoglycoprotein while the monolayers showed no indication of staining. Moreover, mucin-specific antibodies to MUC1 and MUC5A bound with greater affinity to 3-D aggregates than to the monolayers. P. aeruginosa attached to and penetrated A549 monolayers significantly more than the same cells grown as 3-D aggregates. Scanning electron microscopy of A549 cells grown as monolayers and 3-D aggregates infected with P. aeruginosa showed that monolayers detached from the surface of the culture plate postinfection, in contrast to the 3-D aggregates, which remained attached to the microcarrier beads. In response to infection, proinflammatory cytokine levels were elevated for the 3-D A549 aggregates compared to monolayer controls. These findings suggest that A549 lung cells grown as 3-D aggregates may represent a more physiologically relevant model to examine the interactions between P. aeruginosa and the lung epithelium during infection.

Original languageEnglish (US)
Pages (from-to)1129-1140
Number of pages12
JournalInfection and Immunity
Volume73
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

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Pseudomonas aeruginosa
Epithelial Cells
Lung
Electron Scanning Microscopy
Infection
Immunohistochemistry
Cytokines
Somatostatin-Secreting Cells
Tight Junctions
Bioreactors
Mucins
Epithelium
Staining and Labeling
Antibodies
Neoplasms
A549 Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Carterson, A. J., Höner Zu Bentrup, K., Ott, C. M., Clarke, M. S., Pierson, D. L., Vanderburg, C. R., ... Schurr, M. J. (2005). A549 lung epithelial cells grown as three-dimensional aggregates: Alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. Infection and Immunity, 73(2), 1129-1140. https://doi.org/10.1128/IAI.73.2.1129-1140.2005

A549 lung epithelial cells grown as three-dimensional aggregates : Alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. / Carterson, A. J.; Höner Zu Bentrup, K.; Ott, C. M.; Clarke, M. S.; Pierson, D. L.; Vanderburg, C. R.; Buchanan, K. L.; Nickerson, Cheryl; Schurr, M. J.

In: Infection and Immunity, Vol. 73, No. 2, 02.2005, p. 1129-1140.

Research output: Contribution to journalArticle

Carterson, AJ, Höner Zu Bentrup, K, Ott, CM, Clarke, MS, Pierson, DL, Vanderburg, CR, Buchanan, KL, Nickerson, C & Schurr, MJ 2005, 'A549 lung epithelial cells grown as three-dimensional aggregates: Alternative tissue culture model for Pseudomonas aeruginosa pathogenesis', Infection and Immunity, vol. 73, no. 2, pp. 1129-1140. https://doi.org/10.1128/IAI.73.2.1129-1140.2005
Carterson, A. J. ; Höner Zu Bentrup, K. ; Ott, C. M. ; Clarke, M. S. ; Pierson, D. L. ; Vanderburg, C. R. ; Buchanan, K. L. ; Nickerson, Cheryl ; Schurr, M. J. / A549 lung epithelial cells grown as three-dimensional aggregates : Alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. In: Infection and Immunity. 2005 ; Vol. 73, No. 2. pp. 1129-1140.
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