A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy

Luke C M Mackinder, Charlotte A. Worthy, Gaia Biggi, Matthew Hall, Keith P. Ryan, Arvind Varsani, Glenn M. Harper, William H. Wilson, Colin Brownlee, Declan C. Schroeder

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Emiliania huxleyi virus 86 (EhV-86) belongs to the family Phycodnaviridae, a group of viruses that infect a wide range of freshwater and marine eukaryotic algae. Phycodnaviridae is one of the five families that belong to a large and phylogenetically diverse group of viruses known as nucleocytoplasmic large dsDNA viruses (NCLDVs). To date, our understanding of algal NCLDV entry is based on the entry mechanisms of members of the genera Chlorovirus and Phaeovirus, both of which consist of non-enveloped viruses that 'inject' their genome into their host via a viral inner-membrane host plasma membrane fusion mechanism, leaving an extracellular viral capsid. Using a combination of confocal and electron microscopy, this study demonstrated for the first time that EhV-86 differs from its algal virus counterparts in two fundamental areas. Firstly, its capsid is enveloped by a lipid membrane, and secondly, EhV-86 enters its host via either an endocytotic or an envelope fusion mechanism in which an intact nucleoprotein core still encapsulated by its capsid is seen in the host cytoplasm. Real-time fluorescence microscopy showed that viral internalization and virion breakdown took place within the host on a timescale of seconds. At around 4.5 h post-infection, virus progeny were released via a budding mechanism during which EhV-86 virions became enveloped with host plasma membrane. EhV-86 therefore appears to have an infection mechanism different from that employed by other algal NCLDVs, with entry and exit strategies showing a greater analogy to animal-like NCLDVs.

Original languageEnglish (US)
Pages (from-to)2306-2316
Number of pages11
JournalJournal of General Virology
Volume90
Issue number9
DOIs
StatePublished - 2009
Externally publishedYes

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Phycodnaviridae
Haptophyta
Viruses
Infection
Capsid
Virus Internalization
Virion
Cell Membrane
Membrane Fusion
Nucleoproteins
Virus Diseases
Membrane Lipids
Fresh Water
Fluorescence Microscopy
Confocal Microscopy

ASJC Scopus subject areas

  • Medicine(all)
  • Virology

Cite this

Mackinder, L. C. M., Worthy, C. A., Biggi, G., Hall, M., Ryan, K. P., Varsani, A., ... Schroeder, D. C. (2009). A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy. Journal of General Virology, 90(9), 2306-2316. https://doi.org/10.1099/vir.0.011635-0

A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy. / Mackinder, Luke C M; Worthy, Charlotte A.; Biggi, Gaia; Hall, Matthew; Ryan, Keith P.; Varsani, Arvind; Harper, Glenn M.; Wilson, William H.; Brownlee, Colin; Schroeder, Declan C.

In: Journal of General Virology, Vol. 90, No. 9, 2009, p. 2306-2316.

Research output: Contribution to journalArticle

Mackinder, LCM, Worthy, CA, Biggi, G, Hall, M, Ryan, KP, Varsani, A, Harper, GM, Wilson, WH, Brownlee, C & Schroeder, DC 2009, 'A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy', Journal of General Virology, vol. 90, no. 9, pp. 2306-2316. https://doi.org/10.1099/vir.0.011635-0
Mackinder, Luke C M ; Worthy, Charlotte A. ; Biggi, Gaia ; Hall, Matthew ; Ryan, Keith P. ; Varsani, Arvind ; Harper, Glenn M. ; Wilson, William H. ; Brownlee, Colin ; Schroeder, Declan C. / A unicellular algal virus, Emiliania huxleyi virus 86, exploits an animal-like infection strategy. In: Journal of General Virology. 2009 ; Vol. 90, No. 9. pp. 2306-2316.
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AU - Ryan, Keith P.

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