A turn-off fluorescent substrate for horseradish peroxidase improves the sensitivity of ELISAs

Abhinav P. Acharya, Payel Sen, Kiana Aran, Austin B. Gardner, Mohammad Rafi, Deborah Dean, Niren Murthy

Research output: Contribution to journalArticle

Abstract

A new turn-off fluorescent substrate for horseradish peroxidase is introduced, termed clics, which enhances the sensitivity of commercial enzyme-linked immunosorbant assays (ELISA) by 32-fold and detects chlamydial infections in patients. Clics are cost-effective, stable, and can be easily incorporated in commercial ELISA kits, and therefore should have a great impact in the fields of medicine and biology.

Original languageEnglish (US)
Pages (from-to)206-210
Number of pages5
JournalJournal of Polymer Science, Part A: Polymer Chemistry
Volume53
Issue number2
DOIs
StatePublished - Jan 15 2015
Externally publishedYes

Keywords

  • ELISA
  • biomaterials
  • chlamydia
  • cyanine
  • diagnostics
  • enzymes
  • liposome
  • nanotechnology
  • ultrasensitive

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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