A systematic exploration of the interactions between bacterial effector proteins and host cell membranes

Bethany Weigele, Robert C. Orchard, Alyssa Jimenez, Gregory W. Cox, Neal M. Alto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Membrane-bound organelles serve as platforms for the assembly of multi-protein complexes that function as hubs of signal transduction in eukaryotic cells. Microbial pathogens have evolved virulence factors that reprogram these host signaling responses, but the underlying molecular mechanisms are poorly understood. Here we test the ability of ~200 type III and type IV effector proteins from six Gram-negative bacterial species to interact with the eukaryotic plasma membrane and intracellular organelles. We show that over 30% of the effectors localize to yeast and mammalian cell membranes, including a subset of previously uncharacterized Legionella effectors that appear to be able to regulate yeast vacuolar fusion. A combined genetic, cellular, and biochemical approach supports that some of the tested bacterial effectors can bind to membrane phospholipids and may regulate membrane trafficking. Finally, we show that the type III effector IpgB1 from Shigella flexneri may bind to acidic phospholipids and regulate actin filament dynamics.

Original languageEnglish (US)
Article number532
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

Fingerprint

effectors
Bacterial Proteins
Cell membranes
Cell Membrane
proteins
Membranes
Organelles
Yeast
Phospholipids
membranes
Yeasts
organelles
yeast
Shigella flexneri
Legionella
Signal transduction
Proteins
interactions
Eukaryotic Cells
Virulence Factors

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

A systematic exploration of the interactions between bacterial effector proteins and host cell membranes. / Weigele, Bethany; Orchard, Robert C.; Jimenez, Alyssa; Cox, Gregory W.; Alto, Neal M.

In: Nature Communications, Vol. 8, No. 1, 532, 01.12.2017.

Research output: Contribution to journalArticle

Weigele, Bethany ; Orchard, Robert C. ; Jimenez, Alyssa ; Cox, Gregory W. ; Alto, Neal M. / A systematic exploration of the interactions between bacterial effector proteins and host cell membranes. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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