A structural model of the active ribosome-bound membrane protein insertase YidC

Stephan Wickles, Abhishek Singharoy, Jessica Andreani, Stefan Seemayer, Lukas Bischoff, Otto Berninghausen, Johannes Soeding, Klaus Schulten, Eli O. van der Sluis, Roland Beckmann

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The integration of most membrane proteins into the cytoplasmic membrane of bacteria occurs co-translationally. The universally conserved YidC protein mediates this process either individually as a membrane protein insertase, or in concert with the SecY complex. Here, we present a structural model of YidC based on evolutionary co-variation analysis, lipid-versus-protein-exposure and molecular dynamics simulations. The model suggests a distinctive arrangement of the conserved five transmembrane domains and a helical hairpin between transmembrane segment 2 (TM2) and TM3 on the cytoplasmic membrane surface. The model was used for docking into a cryo-electron microscopy reconstruction of a translating YidC-ribosome complex carrying the YidC substrate FOc. This structure reveals how a single copy of YidC interacts with the ribosome at the ribosomal tunnel exit and identifies a site for membrane protein insertion at the YidC protein-lipid interface. Together, these data suggest a mechanism for the co-translational mode of YidC-mediated membrane protein insertion.

Original languageEnglish (US)
Article numbere03035
Pages (from-to)1-17
Number of pages17
JournaleLife
Volume3
Issue numberJuly2014
DOIs
StatePublished - Jul 10 2014
Externally publishedYes

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Structural Models
Ribosomes
Membrane Proteins
Cell Membrane
Membranes
Cryoelectron Microscopy
Lipids
Proteins
Molecular Dynamics Simulation
Electron microscopy
Molecular dynamics
Bacteria
Tunnels
Computer simulation
Substrates

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wickles, S., Singharoy, A., Andreani, J., Seemayer, S., Bischoff, L., Berninghausen, O., ... Beckmann, R. (2014). A structural model of the active ribosome-bound membrane protein insertase YidC. eLife, 3(July2014), 1-17. [e03035]. https://doi.org/10.7554/eLife.03035

A structural model of the active ribosome-bound membrane protein insertase YidC. / Wickles, Stephan; Singharoy, Abhishek; Andreani, Jessica; Seemayer, Stefan; Bischoff, Lukas; Berninghausen, Otto; Soeding, Johannes; Schulten, Klaus; van der Sluis, Eli O.; Beckmann, Roland.

In: eLife, Vol. 3, No. July2014, e03035, 10.07.2014, p. 1-17.

Research output: Contribution to journalArticle

Wickles, S, Singharoy, A, Andreani, J, Seemayer, S, Bischoff, L, Berninghausen, O, Soeding, J, Schulten, K, van der Sluis, EO & Beckmann, R 2014, 'A structural model of the active ribosome-bound membrane protein insertase YidC', eLife, vol. 3, no. July2014, e03035, pp. 1-17. https://doi.org/10.7554/eLife.03035
Wickles S, Singharoy A, Andreani J, Seemayer S, Bischoff L, Berninghausen O et al. A structural model of the active ribosome-bound membrane protein insertase YidC. eLife. 2014 Jul 10;3(July2014):1-17. e03035. https://doi.org/10.7554/eLife.03035
Wickles, Stephan ; Singharoy, Abhishek ; Andreani, Jessica ; Seemayer, Stefan ; Bischoff, Lukas ; Berninghausen, Otto ; Soeding, Johannes ; Schulten, Klaus ; van der Sluis, Eli O. ; Beckmann, Roland. / A structural model of the active ribosome-bound membrane protein insertase YidC. In: eLife. 2014 ; Vol. 3, No. July2014. pp. 1-17.
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