A strategy for using multiple linked markers for genetic counseling

A. Chakravarti, Kenneth Buetow

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A strategy for using multiple linked markers for genetic counseling is to test sequentially individual markers until a diagnosis can be made. We show that in order to minimize the number of tests performed per case while diagnosing all informative cases the order in which the markers are to be tested is critical. We describe an algorithm to obtain this order using the parameter 'I', the frequency of informative cases. The I value for a specific locus used depends on the marker frequency, association with the disease locus, and also on the informativeness of the marker loci already tested. Realizing that a direct assay for the β(S) gene already exists, and that most cases of β-thalassemia in Mediterraneans can be directly diagnosed using synthetic oligonucleotide probes, we illustrate the above technique by examining nine DNA polymorphisms in the human β-globin cluster for their ability to diagnose sickle-cell anemia in American blacks and β-thalassemia in Mediterraneans. This analysis shows that 95.39% of all sickle-cell pregnancies can be diagnosed by testing a subset of only six markers chosen by our algorithm. Furthermore, six markers can also diagnose 88.03% of β-thalassemia in Greeks and 83.56% of β-thalassemia in Italians. The test set is different from that suggested by the individual informative frequencies due to nonrandom associations between the restriction sites.

Original languageEnglish (US)
Pages (from-to)984-997
Number of pages14
JournalAmerican Journal of Human Genetics
Volume37
Issue number5
StatePublished - 1985
Externally publishedYes

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Thalassemia
Genetic Counseling
Globins
Oligonucleotide Probes
Sickle Cell Anemia
Pregnancy
DNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

A strategy for using multiple linked markers for genetic counseling. / Chakravarti, A.; Buetow, Kenneth.

In: American Journal of Human Genetics, Vol. 37, No. 5, 1985, p. 984-997.

Research output: Contribution to journalArticle

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