TY - JOUR
T1 - A Species-Specific Repetitive Sequence in Mycobacterium Leprae DNA
AU - Clark-Curtiss, Josephine E.
AU - Docherty, Martin A.
N1 - Funding Information:
Received for publication 13 April 1988 and in revised form 3 August 1988. This work was supported by the Chemotherapy of Leprosy and the Immunology of Leprosy components of the United Nations Development Program/World Bank/World Health Organization Special Programme for Research and Training in Tropical Dis-eases and by grant AI-23470 from the National Institute of Al lergy and Infectious Diseases. Wethank Drs. G. P. Walsh (Armed Forces Institute of Pathology, Washington, D. C.), T. P. Gillis (G. W. Long Hansen's Disease Research Center, Carville, Louisiana), and the late C. C. Shepard (CDC, Atlanta) for providing infected armadillo liver tissue; Drs. B. R. Bloom and C. Grosskinsky (Albert Einstein College of Medicine, New York) for chromosomal DNA from M. avium, M bovis BCG, M. chelonei, M jortuitum, M. intracellulare, M. microti, and M. tuberculosis; and Drs. Gary P. Weil (Washington University), V. N. Bhatia, P. N. Neelan, V. Ramachandran, and N. Muthaya and V. Ramanayya (Central Leprosy Teaching and Research Institute, Chingleput, India) for skin biopsy material from patients with leprosy. Please address requests for reprints to Dr. Josephine E. ClarkCurtiss, Department of Biology, Box 1137,Washington University, St. Louis, Missouri 63130. * Present address: St. Louis University School of Medicine, 51. Louis, Missouri.
PY - 1989/1
Y1 - 1989/1
N2 - A 2.2-kilobase Mycobacterium leprae DNA insert fragment from a recombinant genomic library (pYA1065) was found to hybridize to at least 19 fragments of chromosomal M leprae DNA by Southern hybridizations. The probe hybridized to identical fragments of chromosomal DNA from four M. leprae isolates (two from patients with leprosy, one from a naturally infected armadillo, and one from a naturally infected Mangabey monkey) whether the chromosomal DNA was digested with BamHI, Bst/EII, PstI, or SacI. The pYA1065 probe is specific for M. leprae; it did not hybridize to chromosomal DNA from 14 cultivable slow- and fast-growing mycobacterial species. Dot-blot hybridizations between pYA1065 and purified M. leprae chromosomal DNA indicate that the probe can detect DNA equivalent to 4 Ü 103 M. leprae cells in a spot. The probe can also hybridize to DNA in M. leprae cells spotted on a filter from homogenized skin biopsy specimens from patients with lepromatous leprosy.
AB - A 2.2-kilobase Mycobacterium leprae DNA insert fragment from a recombinant genomic library (pYA1065) was found to hybridize to at least 19 fragments of chromosomal M leprae DNA by Southern hybridizations. The probe hybridized to identical fragments of chromosomal DNA from four M. leprae isolates (two from patients with leprosy, one from a naturally infected armadillo, and one from a naturally infected Mangabey monkey) whether the chromosomal DNA was digested with BamHI, Bst/EII, PstI, or SacI. The pYA1065 probe is specific for M. leprae; it did not hybridize to chromosomal DNA from 14 cultivable slow- and fast-growing mycobacterial species. Dot-blot hybridizations between pYA1065 and purified M. leprae chromosomal DNA indicate that the probe can detect DNA equivalent to 4 Ü 103 M. leprae cells in a spot. The probe can also hybridize to DNA in M. leprae cells spotted on a filter from homogenized skin biopsy specimens from patients with lepromatous leprosy.
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U2 - 10.1093/infdis/159.1.7
DO - 10.1093/infdis/159.1.7
M3 - Article
C2 - 2642523
AN - SCOPUS:0024538061
SN - 0022-1899
VL - 159
SP - 7
EP - 15
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -