A species-specific repetitive sequence in Mycobacterium leprae DNA

J. E. Clark-Curtiss, M. A. Docherty

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A 2.2-kilobase Mycobacterium leprae DNA insert fragment from a recombinant genomic library (pYA1065) was found to hybridize to at least 19 fragments of chromosomal M. leprae DNA by Southern hybridizations. The probe hybridized to identical fragments of chromosomal DNA from four M. leprae isolates (two from patients with leprosy, one from a naturally infected armadillo, and one from a naturally infected Mangabey monkey) whether the chromosomal DNA was digested with BamHI, BstEII, PstI, or SacI. The pYA1065 probe is specific for M. leprae; it did not hybridize to chromosomal DNA from 14-cultivable slow- and fast-growing mycobacterial species. Dot-blot hybridizations between pYA1065 and purified M. leprae chromosomal DNA indicate that the probe can detect DNA equivalent to 4 x 103 M. leprae cells in a spot. The probe can also hybridize to DNA in M. leprae cells spotted on a filter from homogenized skin biopsy specimens from patients with lepromatous leprosy.

Original languageEnglish (US)
Pages (from-to)7-15
Number of pages9
JournalJournal of Infectious Diseases
Volume159
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Mycobacterium leprae
Nucleic Acid Repetitive Sequences
DNA
Cercocebus
Armadillos
Lepromatous Leprosy
Genomic Library
DNA Probes
Leprosy
Haplorhini
Biopsy
Skin

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Clark-Curtiss, J. E., & Docherty, M. A. (1989). A species-specific repetitive sequence in Mycobacterium leprae DNA. Journal of Infectious Diseases, 159(1), 7-15.

A species-specific repetitive sequence in Mycobacterium leprae DNA. / Clark-Curtiss, J. E.; Docherty, M. A.

In: Journal of Infectious Diseases, Vol. 159, No. 1, 1989, p. 7-15.

Research output: Contribution to journalArticle

Clark-Curtiss, JE & Docherty, MA 1989, 'A species-specific repetitive sequence in Mycobacterium leprae DNA', Journal of Infectious Diseases, vol. 159, no. 1, pp. 7-15.
Clark-Curtiss, J. E. ; Docherty, M. A. / A species-specific repetitive sequence in Mycobacterium leprae DNA. In: Journal of Infectious Diseases. 1989 ; Vol. 159, No. 1. pp. 7-15.
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