A spatial control for correct timing of gene expression during the Escherichia coli cell cycle

Yuan Yao, Lifei Fan, Yixin Shi, Ingvild Odsbu, Morigen

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Temporal transcriptions of genes are achieved by different mechanisms such as dynamic interaction of activator and repressor proteins with promoters, and accumulation and/or degradation of key regulators as a function of cell cycle. We find that the TorR protein localizes to the old poles of the Escherichia coli cells, forming a functional focus. The TorR focus co-localizes with the nucleoid in a cell-cycle-dependent manner, and consequently regulates transcription of a number of genes. Formation of one TorR focus at the old poles of cells requires interaction with the MreB and DnaK proteins, and ATP, suggesting that TorR delivery requires cytoskeleton organization and ATP. Further, absence of the protein-protein interactions and ATP leads to loss in function of TorR as a transcription factor. We propose a mechanism for timing of cell-cycle-dependent gene transcription, where a transcription factor interacts with its target genes during a specific period of the cell cycle by limiting its own spatial distribution.

Original languageEnglish (US)
Article number1
JournalGenes
Volume8
Issue number1
DOIs
StatePublished - Jan 1 2017

Keywords

  • Cell-cycle-dependent
  • Co-localization
  • Gene expression
  • Nucleoid
  • Spatial control
  • TorR focus

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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