A sopB deletion mutation enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated Salmonella enterica Vaccines

Yuhua Li, Shifeng Wang, Wei Xin, Giorgio Scarpellini, Zhaoxing Shi, Bronwyn Gunn, Kenneth L. Roland, Roy Curtiss

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

SopB is a virulence factor of Salmonella encoded by SPI-5. Salmonella sopB deletion mutants are impaired in their ability to cause local inflammatory responses and fluid secretion into the intestinal lumen and also can enhance the immunogenicity of a vectored antigen. In this study, we evaluated the effects on immunogenicity and the efficacy of a sopB deletion mutation on two Salmonella enterica serovar Typhimurium vaccine strains with different attenuating mutations expressing a highly antigenic α-helical region of the Streptococcus pneumoniae surface protein PspA from an Asd+-balanced lethal plasmid. After oral administration to mice, the two pairs of strains induced high levels of serum antibodies specific for PspA as well as to Salmonella antigens. The levels of antigen-specific serum immunoglobulin G (IgG) and mucosal IgA were higher in mice immunized with sopB mutants. Enzyme-linked immunospot assay results indicated that the spleen cells from mice immunized with a sopB mutant showed higher interleukin-4 and gamma interferon secretion levels than did the mice immunized with the isogenic sopB+ strain. The sopB mutants also induced higher numbers of CD4+ CD44 hi CD62Lhi and CD8+ CD44hi CD62Lhi central memory T cells. Eight weeks after primary oral immunization, mice were challenged with 100 50% lethal doses of virulent S. pneumoniae WU2. Immunization with either of the sopB mutant strains led to increased levels of protection compared to that with the isogenic sopB + parent. Together, these results demonstrate that the deletion of sopB leads to an overall enhancement of the immunogenicity and efficacy of recombinant attenuated Salmonella vaccine strains.

Original languageEnglish (US)
Pages (from-to)5238-5246
Number of pages9
JournalInfection and Immunity
Volume76
Issue number11
DOIs
StatePublished - Nov 2008

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Salmonella Vaccines
Heterophile Antigens
Attenuated Vaccines
Salmonella enterica
Sequence Deletion
Salmonella
Streptococcus pneumoniae
Antigens
Immunization
Fluids and Secretions
Enzyme-Linked Immunospot Assay
Synthetic Vaccines
Lethal Dose 50
Virulence Factors
Serum
Interleukin-4
Immunoglobulin A
Interferon-gamma
Oral Administration
Membrane Proteins

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

A sopB deletion mutation enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated Salmonella enterica Vaccines. / Li, Yuhua; Wang, Shifeng; Xin, Wei; Scarpellini, Giorgio; Shi, Zhaoxing; Gunn, Bronwyn; Roland, Kenneth L.; Curtiss, Roy.

In: Infection and Immunity, Vol. 76, No. 11, 11.2008, p. 5238-5246.

Research output: Contribution to journalArticle

Li, Yuhua ; Wang, Shifeng ; Xin, Wei ; Scarpellini, Giorgio ; Shi, Zhaoxing ; Gunn, Bronwyn ; Roland, Kenneth L. ; Curtiss, Roy. / A sopB deletion mutation enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated Salmonella enterica Vaccines. In: Infection and Immunity. 2008 ; Vol. 76, No. 11. pp. 5238-5246.
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