Abstract
Euglenozoa comprises euglenids, kinetoplastids, and diplonemids, with each group exhibiting different and highly unusual mitochondrial genome organizations. Although they are sister groups, kinetoplastids and diplonemids have very distinct mitochondrial genome architectures, requiring widespread insertion/deletion RNA editing and extensive trans-splicing, respectively, in order to generate functional transcripts. The evolutionary history by which these differing processes arose remains unclear. Using single-cell genomics, followed by small sub unit ribosomal DNA and multigene phylogenies, we identified an isolated marine cell that branches on phylogenetic trees as a sister to known kinetoplastids. Analysis of singlecell amplified genomic material identified multiple mitochondrial genome contigs. These revealed a gene architecture resembling that of diplonemid mitochondria, with small fragments of genes encoded out of order and or on different contigs, indicating that these genes require extensive trans-splicing. Conversely, no requirement for kinetoplastid-like insertion/deletion RNA-editing was detected. Additionally, while we identified some proteins so far only found in kinetoplastids, we could not unequivocally identify mitochondrial RNA editing proteins. These data invite the hypothesis that extensive genome fragmentation and trans-splicing were the ancestral states for the kinetoplastid-diplonemid clade but were lost during the kinetoplastid radiation. This study demonstrates that single-cell approaches can successfully retrieve lineages that represent important new branches on the tree of life, and thus can illuminate major evolutionary and functional transitions in eukaryotes.
Original language | English (US) |
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Article number | 20190100 |
Journal | Philosophical Transactions of the Royal Society B: Biological Sciences |
Volume | 374 |
Issue number | 1786 |
DOIs | |
State | Published - Nov 25 2019 |
Keywords
- Diplonemids
- Evolution
- Kinetoplastids
- Mitochondrial genome
- Single-cell genomics
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
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Table S2 from A single-cell genome reveals diplonemid-like ancestry of kinetoplastid mitochondrial gene structure
Wideman, J. (Creator), Lax, G. (Creator), Leonard, G. (Creator), Milner, D. S. (Creator), Rodríguez-Martínez, R. (Creator), Simpson, A. G. B. (Creator) & Richards, T. A. (Creator), figshare Academic Research System, 2019
DOI: 10.6084/m9.figshare.9736241.v1, https://rs.figshare.com/articles/Table_S2_from_A_single-cell_genome_reveals_diplonemid-like_ancestry_of_kinetoplastid_mitochondrial_gene_structure/9736241/1
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Fig. S3 - ESOM.pdf from A single-cell genome reveals diplonemid-like ancestry of kinetoplastid mitochondrial gene structure
Wideman, J. (Contributor), Richards, T. A. (Contributor), Simpson, A. G. B. (Contributor), Rodríguez-Martínez, R. (Contributor), Milner, D. S. (Contributor), Leonard, G. (Contributor) & Lax, G. (Contributor), figshare Academic Research System, Jan 1 2019
DOI: 10.6084/m9.figshare.9736232.v1, https://doi.org/10.6084%2Fm9.figshare.9736232.v1
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Supplementary material from "A single-cell genome reveals diplonemid-like ancestry of kinetoplastid mitochondrial gene structure"
Wideman, J. (Creator), Lax, G. (Creator), Leonard, G. (Creator), Milner, D. S. (Creator), Rodríguez-Martínez, R. (Creator), Simpson, A. G. B. (Creator) & Richards, T. A. (Creator), figshare Academic Research System, 2019
DOI: 10.6084/m9.figshare.c.4645130.v1, https://rs.figshare.com/collections/Supplementary_material_from_A_single-cell_genome_reveals_diplonemid-like_ancestry_of_kinetoplastid_mitochondrial_gene_structure_/4645130/1
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